Heterogeneous expression of stearoyl-acyl carrier protein desaturase genes SAD1 and SAD2 from Linum usitatissimum enhances seed oleic acid accumulation and seedling cold and drought tolerance in Brassica napus  

作　　者：Jianjun Wang Yanan Shao Xin Yang Chi Zhang Yuan Guo Zijin Liu Mingxun Chen 

机构地区：[1]State Key Laboratory of Crop Stress Biology for Arid Areas/National Yangling Agricultural Biotechnology&Breeding Center/Shaanxi Key Laboratory of Crop Heterosis/College of Agronomy,Northwest A&F University,Yangling 712100,China

出　　处：《Journal of Integrative Agriculture》2024年第6期1864-1878,共15页农业科学学报（英文版）

基　　金：supported by the National Science and Technology Innovation 2030 of China(2022ZD04010);the National Key Research and Development Program of China(2022YFD1200400);the Key Research and Development Program of Shaanxi Province,China(2022NY-158);the Ph D Start-up Fund of Northwest A&F University,China(Z1090121052);a grant from the Yang Ling Seed Industry Innovation Center,China(K3031122024).

摘　　要：Flax(Linum usitatissimum L.)is a versatile crop and its seeds are a major source of unsaturated fatty acids.Stearoyl-acyl carrier protein desaturase(SAD)is a dehydrogenase enzyme that plays a key role in oleic acid biosynthesis as well as responses to biotic and abiotic stresses.However,the function of SAD orthologs from L.usitatissimum has not been assessed.Here,we found that two LuSAD genes,LuSAD1 and LuSAD2,are present in the genome of L.usitatissimum cultivar‘Longya 10’.Heterogeneous expression of either LuSAD1 or LuSAD2 in Arabidopsis thaliana resulted in higher contents of total fatty acids and oleic acid in the seeds.Interestingly,ectopic expression of LuSAD2 in A.thaliana caused altered plant architecture.Similarly,the overexpression of either LuSAD1 or LuSAD2 in Brassica napus also resulted in increased contents of total fatty acids and oleic acid in the seeds.Furthermore,we demonstrated that either LuSAD1 or LuSAD2 enhances seedling resistance to cold and drought stresses by improving antioxidant enzyme activity and nonenzymatic antioxidant levels,as well as reducing membrane damage.These findings not only broaden our knowledge of the LuSAD functions in plants,but also offer promising targets for improving the quantity and quality of oil,and the abiotic stress tolerance of oil-producing crops,through molecular manipulation.

关 键 词：LuSAD oleic acid cold tolerance drought tolerance Linum usitatissimum Brassica napus 

分 类 号：S565.4[农业科学—作物学]