Eureka lemon zinc finger protein ClDOF3.4 interacts with citrus yellow vein clearing virus coat protein to inhibit viral infection  

作　　者：Ping Liao Ting Zeng Mengyang Huangfu Cairong Zheng Jiequn Ren Changyong Zhou Yan Zhou 

机构地区：[1]Integrative Science Center of Germplasm Creation in Western China(CHONGQING)Science City,Citrus Research Institute,Southwest University,Chongqing 400712,China [2]National Citrus Engineering Research Center,Citrus Research Institute,Southwest University,Chongqing 400712,China [3]Chongqing Three Gorges Academy of Agricultural Science,Chongqing Three Gorges University,Chongqing 404020,China

出　　处：《Journal of Integrative Agriculture》2024年第6期1979-1993,共15页农业科学学报（英文版）

基　　金：supported by the China Agriculture Research System of MOF and MARA(CARS26-05B);the Innovation Research 2035 Pilot Plan of Southwest University,China(SWU-XDPY22002);the Guangxi Science and Technology Planed Project,China(Gui Ke AD23026090);the Guangxi Natural Science Foundation,China(2023GXNSFBA026285).

摘　　要：Citrus yellow vein clearing virus(CYVCV)is a new citrus virus that has become an important factor restricting the development of China’s citrus industry,and the CYVCV coat protein(CP)is associated with viral pathogenicity.In this study,the Eureka lemon zinc finger protein(ZFP)ClDOF3.4 was shown to interact with CYVCV CP in vivo and in vitro.Transient expression of ClDOF3.4 in Eureka lemon induced the expression of salicylic acid(SA)-related and hypersensitive response marker genes,and triggered a reactive oxygen species burst,ion leakage necrosis,and the accumulation of free SA.Furthermore,the CYVCV titer in ClDOF3.4 transgenic Eureka lemon plants was approximately 69.4%that in control plants 6 mon after inoculation,with only mild leaf chlorotic spots observed in those transgenic plants.Taken together,the results indicate that ClDOF3.4 not only interacts with CP but also induces an immune response in Eureka lemon by inducing the SA pathways.This is the first report that ZFP is involved in the immune response of a citrus viral disease,which provides a basis for further study of the molecular mechanism of CYVCV infection.

关 键 词：citrus yellow vein clearing virus coat protein ClDOF3.4 plant immune response 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]