Effects of long-term partial substitution of inorganic fertilizer with pig manure and/or straw on nitrogen fractions and microbiological properties in greenhouse vegetable soils  

作　　者：Shuo Yuan Ruonan Li Yinjie Zhang Hao'an Luan Jiwei Tang Liying Wang Hongjie Ji Shaowen Huang 

机构地区：[1]State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China/Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081,China [2]Institute of Agricultural Resources and Environment,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050051,China [3]College of Resources and Environment,Henan Agricultural University,Zhengzhou 450002,China [4]College of Forestry,Hebei Agricultural University,Baoding 071000,China

出　　处：《Journal of Integrative Agriculture》2024年第6期2083-2098,共16页农业科学学报（英文版）

基　　金：supported by the earmarked fund for China Agriculture Research System(CARS-23-B04);the National Key Research and Development Program of China(2016YFD0201001);HAAFS Science and Technology Innovation Special Project,China(2022KJCXZX-ZHS-2).

摘　　要：Partial substitution of inorganic fertilizers with organic amendments is an important agricultural management practice.An 11-year field experiment(22 cropping periods)was carried out to analyze the impacts of different partial substitution treatments on crop yields and the transformation of nitrogen fractions in greenhouse vegetable soil.Four treatments with equal N,P_(2)O_(5),and K_(2)O inputs were selected,including complete inorganic fertilizer N(CN),50%inorganic fertilizer N plus 50%pig manure N(CPN),50%inorganic fertilizer N plus 25%pig manure N and 25%corn straw N(CPSN),and 50%inorganic fertilizer N plus 50%corn straw N(CSN).Organic substitution treatments tended to increase crop yields since the 6th cropping period compared to the CN treatment.From the 8th to the 22nd cropping periods,the highest yields were observed in the CPSN treatment where yields were 7.5-11.1%greater than in CN treatment.After 11-year fertilization,compared to CN,organic substitution treatments significantly increased the concentrations of NO_(3)^(-)-N,NH_(4)^(+)-N,acid hydrolysis ammonium-N(AHAN),amino acid-N(AAN),amino sugar-N(ASN),and acid hydrolysis unknown-N(AHUN)in soil by 45.0-69.4,32.8-58.1,49.3-66.6,62.0-69.5,34.5-100.3,and 109.2-172.9%,respectively.Redundancy analysis indicated that soil C/N and OC concentration significantly affected the distribution of N fractions.The highest concentrations of NO_(3)^(-)-N,AHAN,AAN,AHUN were found in the CPSN treatment.Organic substitution treatments increased the activities ofβ-glucosidase,β-cellobiosidase,N-acetyl-glucosamidase,L-aminopeptidase,and phosphatase in the soil.Organic substitution treatments reduced vector length and increased vector angle,indicating alleviation of constraints of C and N on soil microorganisms.Organic substitution treatments increased the total concentrations of phospholipid fatty acids(PLFAs)in the soil by 109.9-205.3%,and increased the relative abundance of G^(+)bacteria and fungi taxa,but decreased the relative abundance of G-bacteria,total bacteria,and

关 键 词：organic substitution management greenhouse vegetable production N fractions microbial community composition extracellular enzyme activity enzyme stoichiometry coefficients 

分 类 号：S626[农业科学—园艺学]