肺类器官研究及应用进展  

Progress in research and application of lung organoids

作　　者：杨广平 房贺 刘晓彬 付忠华[1] 朱峰[3] YANG Guangping;FANG He;LIU Xiaobin;FU Zhonghua;ZHU Feng(Medical Center of Burn Plastic and Wound Repair,The First Affiliated Hospital of Nanchang University,Nanchang 330006,China;ICU of Burn and Trauma,the First Hospital,Naval Medical University,Shanghai 200433,China;Department of Critical Care Medicine,Shanghai East Hospital,Tongji University School of Medicine,Shanghai 200433,China)

机构地区：[1]南昌大学第一附属医院烧伤整形与创面修复医学中心,江西南昌330006 [2]海军军医大学第一附属医院烧创伤ICU,上海200433 [3]同济大学附属东方医院重症医学科,上海200120

出　　处：《中国病理生理杂志》2024年第6期1122-1127,1146,共7页Chinese Journal of Pathophysiology

基　　金：国家重点研发计划“干细胞及转化研究”重点专项(No.2019YFA0110601);国家自然科学基金资助项目(No.91210452);上海市科技创新行动计划(No.22Y11900200)。

摘　　要：在以往动物模型的研究中,由于动物与人类肺部解剖结构的差异以及在药物研发中使用动物模型时经常出现令人失望的结果,而2D细胞系难以真实反映人体器官的结构和功能,亟需一种体外模型可以补充动物研究,并提高对肺部生理、再生和疾病等的理解。肺类器官(lung organoids,LO)的诞生跨越了这一鸿沟,实现了由动物模型器官研究到人体器官体外研究的转变。LO是在历经众多实验研究和漫长的经验总结上所研发出来的,为人体肺部的相关研究奠定了基础。In the field of biomedicine,two-dimensional(2D)cell lines and animal models have played an important role in the study of cell pathways and drug targets.However,due to species differences between humans and other animals,and the lack of hierarchy,cellular diversity,and cell-cell or cell-matrix interactions,2D cell lines could not fully reflect what cells actually look like in the human body.Organoids are three-dimensional(3D)in vitro culture models derived from autologous tissue stem cells,which make up for the defects of 2D culture and can simulate the structure and function of real human organs to a certain extent,providing new ideas for disease diagnosis and treatment.Among them,lung organoids(LO)are a typical case studying the development process of human lung and the generation principle of lung diseases.Relevant studies have provided help for the treatment of pulmonary fibrosis,lung cancer,lung injury and other diseases.This paper aims to summarize and analyze the research progress of lung organoids in recent years,and further summarize the application of LO in the diagnosis and treatment of lung diseases.

关 键 词：肺类器官 肺疾病 干细胞 

分 类 号：R318.1[医药卫生—生物医学工程] R563[医药卫生—基础医学] R-33