八仙茶多酚提取工艺优化及抗氧化作用研究  

Optimization of Extraction Process and Antioxidant Effect of Baxian Tea Polyphenols

作　　者：沈俊炳 SHEN Junbing(Zhaoan Institute of Quality Inspection and Metrological Verification,Zhaoan 363500,China)

机构地区：[1]诏安县质量计量检验检测所,福建诏安363500

出　　处：《现代食品》2024年第9期76-81,共6页Modern Food

基　　金：福建省科技特派员基金(闽科农函〔2022〕36号)。

摘　　要：为了优化八仙茶多酚的提取工艺,并探究其抗氧化活性,本文采用超声波辅助溶剂浸提法,以八仙茶多酚提取量为评价指标,通过单因素实验和正交实验筛选最佳的提取工艺参数,同时通过测定八仙茶多酚对DPPH、ABTS自由基的清除率,对八仙茶多酚的抗氧化性进行评价。结果表明,八仙茶多酚的最佳提取参数为料液比1∶25,乙醇体积分数70%,浸提温度50℃,浸提时间60 min,在此参数下八仙茶多酚的提取量为19.92 mg·g^(-1)。抗氧化活性实验表明,八仙茶多酚对DPPH和ABTS自由基清除效果明显,对DPPH自由基的IC50值为0.02 mg·mL^(-1),对ABTS自由基清除效果与维生素C大致相当。结果证明提取八仙茶多酚的方法可靠有效,所提取的八仙茶多酚具有良好的抗氧化活性。In order to optimize the extraction process of polyphenols from Baxian tea and explore its antioxidant activity.This article uses ultrasonic assisted solvent extraction method,with the extraction amount of Baxian tea polyphenols as the evaluation index.The optimal extraction process parameters are screened through single factor experiments and orthogonal experiments.At the same time,the antioxidant activity of Baxian tea polyphenols is evaluated by measuring their scavenging rates on DPPH and ABTS free radicals.The results showed that the optimal extraction parameters of Baxian tea polyphenols were:material liquid ratio of 1∶25,ethanol volume fraction of 70%,extraction temperature of 50℃,extraction time of 60 min,and the resulting extraction amount was 19.92 mg·g^(-1).The antioxidant activity test showed that the scavenging results of Baxian tea polyphenols on DPPH and ABTS radicals were obvious,with IC50 values of 0.02 mg·mL^(-1)for DPPH radicals and roughly equivalent to Vc for ABTS radicals.The results confirmed that the method of Baxian tea polyphenol extraction was reliable and effective,and the extracted Baxian tea polyphenols had good antioxidant activity.

关 键 词：八仙茶 多酚提取 超声波辅助 溶剂浸提法 抗氧化 

分 类 号：TS272.5[农业科学—茶叶生产加工]