矿泉水中粪链球菌的检测能力验证结果与分析  

The Results and Analysis of Testing of Enterococcus faecalis in Mineral Water

作　　者：李发俊 张琼丹 黄佳慧 叶子爵 LI Fajun;ZHANG Qiongdan;HUANG Jiahui;YE Zijue(Dongguan Institution for Food and Drug Control,Dongguan 523808,China)

机构地区：[1]东莞市食品药品检验所,广东东莞523808

出　　处：《现代食品》2024年第9期171-173,180,共4页Modern Food

基　　金：2022年东莞市社会发展科技重点项目(20221800906242)。

摘　　要：为提高实验室对矿泉水中粪链球菌的检测能力,确保其检测数据的准确性和可靠性,先采用国标方法计数,后采用传统微生物鉴定法、全自动微生物鉴定法和基质辅助激光解吸电离飞行时间质谱法(简称MALDI-TOF MS)3种方法对可疑菌进行鉴定。结果表明,3种方法均成功鉴定出粪链球菌,且样品23-A864和23-N843的Z值分别为-0.5和-0.1,满足要求。3种方法相互验证,其中基质辅助激光解吸电离飞行时间质谱法具有耗时短、效率高的优势。In order to improve the laboratory’s ability to detect Enterococcus faecalis in mineral water,ensuring the accuracy and reliability of test data,the national standard method was used to count the suspected bacteria,and three methods of identification were compared:traditional microbiological identification,automatic microbiological identification,and matrix-assisted laser desorption ionization time-of-flight mass spectrometry.Experimental results showed that all three methods successfully identified Enterococcus faecalis,with Z-values of-0.5 and-0.1 for samples 23-A864 and 23-N843,respectively,meeting the requirements.The three methods are mutually verified,and the MALDI-TOF MS has the advantages of short time and high efficiency.

关 键 词：粪链球菌 矿泉水 微生物鉴定 能力验证 基质辅助激光解吸电离飞行时间质谱法(MALDI-TOF MS) 

分 类 号：Q93.331[生物学—微生物学]