单宁酸对脂多糖应激仔猪肝组织的功能、抗氧化能力和炎症应答的影响  

Effects of Tannic Acid on Liver Tissue Function,Antioxidant Ability and Inflammatory Response in Lipopolysaccharide Stressed Piglets

作　　者：周琦璐 刘金松 吴超 杨彩梅 刘玉兰 张瑞强 ZHOU Qilu;LIU Jinsong;WU Chao;YANG Caimei;LIU Yulan;ZHANG Ruiqiang(Zhejiang Provincial Engineering Laboratory for Animal Health Inspection&Internet Technology,Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province,College of Animal Science and Technology,College of Veterinary Medicine,Zhejiang Agricultural and Forestry University,Hangzhou 311300,China;Zhejiang Vegamax Biotechnology Co.Ltd,Vegamax Green Animal Health Products Key Agricultural Enterprise Research Institute,Anji 313300,China)

机构地区：[1]浙江农林大学动物科技学院•动物医学院,浙江省畜禽绿色生态健康养殖应用技术研究重点实验室,动物健康互联网检测技术浙江省工程实验室,杭州311300 [2]惠嘉绿色动物保健品重点农业企业研究院,浙江惠嘉生物科技股份有限公司,安吉313300

出　　处：《畜牧兽医学报》2024年第6期2519-2529,共11页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：浙江省领军型创新创业团队项目(2020R01015);浙江省重点研发计划项目(2021C02008);浙江省尖兵领雁计划项目(2022C02059);杭州市农业和社会发展一般项目(202203B18);浙江省重点农业企业研究院项目(2021Y30004)。

摘　　要：旨在研究断奶仔猪饲粮添加单宁酸(tannic acid, TA)对脂多糖(lipopolysaccharide, LPS)应激断奶仔猪肝组织功能、抗氧化能力、炎症应答反应及相关基因表达的影响。本试验分为两部分。第一部分选择72头体重相近、健康状况良好的28日龄三元杂交(杜×长×大)断奶仔猪,随机分为2组,分别为基础饲粮饲喂组(饲喂基础饲粮)和单宁酸饲喂组(饲喂在基础饲粮中额外添加1 000 mg·kg^(-1)单宁酸的试验饲粮),每组6个重复,每个重复6头猪,试验期为28 d。第二部分于第一部分试验结束时,分别从基础饲粮饲喂组和单宁酸饲喂组各选取12头体重接近平均体重、健康状况良好的56日龄仔猪,将其分为4组,每组6头仔猪。对照组(CON)和LPS应激组(LPS)的仔猪来自第一部分试验的基础饲粮饲喂组;单宁酸组(TA)和单宁酸+LPS组(TA+LPS)的仔猪来自第一部分试验的单宁酸饲喂组。在仔猪56日龄早上8:00,给LPS组和TA+LPS组仔猪腹腔注射1.5 mL浓度为1 mg·mL^(-1)的LPS,给CON组和TA组仔猪腹腔注射1.5 mL生理盐水,注射1.5 h后屠宰采样。结果发现,LPS应激增加了仔猪的肝脏指数,提高了血清中谷草转氨酶(aspartate aminotransferase, AST)和谷丙转氨酶(alanine aminotransferase, ALT)活性,降低了仔猪肝组织过氧化氢酶(catalase, CAT)和总超氧化物歧化酶(total superoxide dismutase, T-SOD)水平,提高了仔猪肝组织基因Kelch-1ike ECH-associated protein-1(Keap1)、Toll-like receptor 4(TLR4)、Myeloid differentiation factor 88(MyD88)、Nod-like receptor protein 3(NLRP3)、Interleukin-6(IL-6)和Tumor necrosis factor-α(TNF-α)的表达水平,降低了Nuclear factor erythroid 2-related factor 2(Nrf2)、NADH Quinone Oxidoreductase 1(NQO1)、Manganese superoxide dismutase(Mn-SOD)和Catalase(CAT)的表达水平(P<0.05)。饲粮中添加TA可降低仔猪血清中AST和ALT水平,提高仔猪肝组织CAT和T-SOD水平,显著影响仔猪肝组织IL-6、IL-10和TNF-α含量,下调仔猪肝组�This study aimed to investigate the effects of tannic acid added to the diet of weaned piglets on liver index,inflammatory response,antioxidant function,and related gene expression in weaned piglets under lipopolysaccharide stress.The trial was divided into two parts.In the first part,a number of 7228-day-old ternary hybrid weaned piglets(Duroc×Landrace×Yorkshire)with similar weight and good health condition were randomly divided into 2 groups with 6 replicates each and 6 piglets per replicate.The basal diet feeding group was fed a basal diet,and the tannin feeding group was fed the basal diet supplemented with 1000 mg·kg^(-1) tannic acid for 28 days.At the end of the first part of the experiment,a total of 12 twelve 56-day-old piglets with the body weight close to average weight were selected from the basal diet feeding group and the tannin feeding group,respectively,and then randomly divided into 4 groups of 6 piglets in each group.The piglets in control group(CON)and LPS stress group(LPS)were from the basal diet-feeding group of the first part of trial;The piglets in tannin group(TA)and the tannin+LPS group(TA+LPS)were from the tannin feeding group of the first part of trial.At 8 am of the day when the piglets are 56-day-old,the piglets in the LPS group and TA+LPS group were injected intraperitoneally with 1.5 mL of LPS at a concentration of 1 mg·mL^(-1),and piglets in CON and TA groups were injected intraperitoneally with 1.5 mL of normal saline.After 1.5 h of injection,piglets were slaughtered and sampled.The results showed that,LPS stress increased the liver index,enhanced serum aspartate aminotransferase(AST)and alanine aminotransferase(ALT)levels,decreased catalase(CAT)and total superoxide dismutase(T-SOD)activities in liver,and enhanced the mRNA levels of Kelch-1ike ECH-associated protein-1(Keap 1),Toll-like receptor(TLR 4),Myeloid differentiation factor 88(MyD 88),Nod-like receptor protein 3(NLRP 3),Interleukin-6(IL-6)and Tumor necrosis factor-α(TNF-α),reduced the mRNA levels of Nuclear factor ery

关 键 词：单宁酸 脂多糖 猪 肝 应激 

分 类 号：S828.5[农业科学—畜牧学]