甘草多糖对LPS诱导小鼠睾丸炎的调节作用  

Regulation of Glycyrrhizol Polysaccharide Alleviate LPS-induced Orchitis in Mice

作　　者：李美艳 邢晓莹 李娜 张椰莉 乔宏萍[1] 武晓英[1] LI Meiyan;XING Xiaoying;LI Na;ZHANG Yeli;QIAO Hongping;WU Xiaoying(College of Biological Sciences and Technology,Taiyuan Normal University,Taiyuan 030000,China;College of Veterinary Medicine,Shanxi Agricultural University,Taigu 030800,China)

机构地区：[1]太原师范学院生物科学与技术学院,太原030000 [2]山西农业大学动物医学学院,太谷030800

出　　处：《畜牧兽医学报》2024年第6期2741-2750,共10页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：山西省基础研究计划项目(202103021223329);山西省优秀博士来晋工作奖励资金科研项目(I0210261);太原师范学院科研启动经费项目。

摘　　要：本研究旨在探讨甘草多糖(glycyrrhiza polysaccharide, GPS)对LPS诱导小鼠睾丸炎的调节作用及其可能机制。将60只8周龄的雄性C57BL/6J小鼠,随机分为6组(每组10只):空白对照组、模型组(LPS组)、阳性对照组(地塞米松组)、GPS低中高剂量组(100、200、400 mg·kg^(-1))。试验前20 d GPS低中高剂量组灌胃给予相应剂量的GPS,其他组灌胃同等剂量的生理盐水,第21天模型组腹腔注射5 mg·kg^(-1) LPS,其他组腹腔注射等量的生理盐水,2 h后,阳性对照组灌胃给予5 mg·kg^(-1)的地塞米松,12 h后,按照动物伦理学标准处死小鼠,收集小鼠血液及睾丸组织。运用HE染色法观察小鼠睾丸组织结构形态学变化,酶联免疫吸附法(enzyme-linked immunosorbent assay, ELISA)检测血清中睾酮激素(testosterone, T)含量及乳酸脱氢酶(lactate dehydrogenase, LDH)水平,南京建成试剂盒检测氧化损伤指标(T-SOD、CAT、GSH)变化,实时荧光定量PCR法检测睾丸组织中紧密连接蛋白(Occludin、ZO-1)、炎症因子(IL-18、IL-1β、IL-6、TNF-α)及细胞焦亡关键因子(caspase-1、NLRP3、GSDMD)mRNA表达变化,Western blot法检测IL-18、IL-1β、NLRP3、GSDMD的蛋白表达水平。结果显示,阳性对照组与GPS中高剂量组可以显著缓解LPS诱导的小鼠睾丸组织结构损伤,增加血清睾酮含量,改善氧化损伤,降低促炎因子mRNA表达水平,并抑制细胞焦亡发生。综上所述,GPS可以有效缓解LPS诱导的睾丸炎损伤,其作用机制可能与改善睾丸组织氧化损伤,抑制细胞焦亡,阻止炎症反应相关。The present study aimed to investigate the regulation and its possible mechanism of glycyrrhiza polysaccharide(GPS)on LPS-induced orchitis in mice.Sixty 8-week-old male C57BL/6J mice were randomly divided into 6 groups(10 mice per group):Blank control group,model group(LPS group),positive control group(dexamethasone group)and GPS low,medium,high dose(100,200,400 mg·kg^(-1))group.On the first 20 days,GPS low,medium,high dose group was given the corresponding dose of GPS by intragastric,and other groups were given the same dose of physiological saline by intragastric.On the 21st day,model group was intraperitoneally injected with 5 mg·kg^(-1) LPS,and other groups were intraperitoneally injected with the same amount of physiological saline,2 h later,the positive control group was given 5 mg·kg^(-1) dexamethasone by intragastric,12 h later,the mice were sacrificed according to animal ethical standards,blood and testicular tissues were collected.The morphological changes of testicular tissue were observed by HE staining,the content of testosterone(T)and the level of lactate dehydrogenase(LDH)in serum were detected by enzyme-linked immunosorbent assay(ELISA),and the changes of oxidative damage indexes(T-SOD,CAT,GSH)were detected by Nanjing Jiancheng Kit.Real-time fluorescence quantitative PCR was used to detect mRNA expression changes of tight junction proteins(Occludin,ZO-1),inflammatory factors(IL-18,IL-1β,IL-6,TNF-α)and key cytokines of pyroptosis(caspase-1,NLRP3,GSDMD)in testis,western blot was used to detect the protein expression of IL-18,IL-1β,NLRP3,GSDMD.The results showed that dexamethasone group and GPS medium,high dose groups could significantly relieve the LPS-induced testicular damage,increase the serum testosterone content,reduce oxidative damage,decrease the mRNA expressions of pro-inflammatory cytokines,and inhibit the occurrence of pyroptosis.In summary,GPS could effectively alleviate LPS-induced orchitis injury,which may related to reducing oxidative damage,inhibiting pyroptosis and preventing i

关 键 词：甘草多糖 LPS 睾丸炎 细胞焦亡 

分 类 号：S853.74[农业科学—临床兽医学]