二肽基肽酶-Ⅳ有机小分子荧光探针的研究进展  

Progress in Dipeptidyl Peptidase-Ⅳ Organic Small Molecule Fluorescent Probes

作　　者：杨政敏[1] 钟甜甜 胡先运[1] 韩忠耀[1] 覃江克 YANG Zhengmin;ZHONG Tiantian;HU Xianyun;HAN Zhongyao;QIN Jiangke(Qiannan Medical College for Nationalities,Duyun 558003,China;School of Chemistry and Pharmaceutical Sciences,Guangxi Normal University,Guilin 541004,China)

机构地区：[1]黔南民族医学高等专科学校,贵州都匀558003 [2]广西师范大学化学与药学学院,广西桂林5410004

出　　处：《广州化学》2024年第3期17-24,I0002,共9页Guangzhou Chemistry

基　　金：国家自然科学基金项目(81860317);贵州省卫生健康委员会科学基金项目(zwkj2021-453);黔南民族医学高等专科学校基金项目(Qnyz2021001,Qnyz2023001)。

摘　　要：二肽基肽酶-IV(DPP-IV)是一种重要的跨膜糖蛋白水解酶,与各种生理过程和疾病密切相关,检测DPP-IV的有机小分子荧光探针被相继报道,是当前研究生物酶检测的热点。首次综述了用于DPP-IV体外和体内检测及成像的3类有机小分子荧光探针,分别是非近红外荧光探针(λ_(em)<650nm)、近红外荧光探针(λ_(em)>650nm)和双光子荧光探针,对比并总结了三种探针的设计原理、结构特点、生物应用情况及其在DPP-IV相关疾病诊断中的应用,并探讨了DPP-IV活性检测在未来发展方向上所面临的挑战。An essential transmembrane glycoprotein hydrolase,dipeptidyl peptidase-IV(DPP-IV)is intimately associated with a number of physiological functions and illnesses.Current research on biological enzyme detection is focused on organic small-molecule fluorescent probes,which have been published one after another for the detection of DPP-IV.For the first time,three types of organic small molecule fluorescent probes used for in vitro and in vivo detection and imaging of DPP-IV,namely,non-near-infrared fluorescent probes(λ_(em)<650 nm),near-infrared fluorescent probes(λ_(em)>650 nm),and two-photon fluorescent probes,were reviewed,and the design principles,structural features,and bioapplications of the three types of probes were compared and summarized,as well as their diagnostic applications in the diagnosis of DPP-IV-related diseases.Its application in the diagnosis of DPP-IV-related diseases,and discusses the challenges of DPP-IV activity detection in the future development direction.

关 键 词：二肽基肽酶-Ⅳ 荧光探针 有机小分子 生物成像 研究进展 

分 类 号：O625[理学—有机化学]