不同肝细胞配体修饰的壳聚糖作为肝组织再生支架材料的比较研究:制备、表征和评价  

Comparative Study on Chitosan Modified with Different Hepatocyte Ligands as Scaffold Material for Liver Tissue Regeneration:Preparation,Characterization and Evaluation

作　　者：王哲[1] 余凡[1] 姜玉新 侯玉婷 帅兰 邓敏 王红声[1] WANG Zhe;YU Fan;JIANG Yuxin;HOU Yuting;SHUAI Lan;DENG Min;WANG Hongsheng(Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine,College of Biological Science and Medical Engineering,Donghua University,Shanghai 201620,China;The Affiliated Hospital of Jiaxing University,Jiaxing Key Laboratory of Virus-Related Infectious Diseases,Jiaxing University,Jiaxing 314001,China)

机构地区：[1]东华大学生物与医学工程学院,上海纳米生物材料与再生医学工程技术研究中心,上海201620 [2]嘉兴大学附属嘉兴市第一医院,嘉兴市病毒相关传染性病重点实验室,浙江嘉兴314001

出　　处：《Journal of Donghua University(English Edition)》2024年第3期263-274,共12页东华大学学报（英文版）

基　　金：Science&Technology Commission of Shanghai Municipality (No. 20DZ2254900);Municipal Public Welfare Research Project from Jiaxing,Zhejiang Province (No. 2022AY10001);Open Project Program of Jiaxing Key Laboratory of Virus-Related Infectious Diseases.

摘　　要：为了获得理想的肝脏再生支架,使用乳酸(lactobionic acid,LA)或/和甘草次酸(glycyrrhetinic acid,GA)对壳聚糖(chitosan,CS)进行改性,得到LA改性的CS(LC)、GA改性的CS(GC)和GA/LA双改性的CS(GLC),并采用绿色静电纺丝法制备了由丝素蛋白(silk fibroin,SF)和上述改性CS组成的复合纳米纤维支架。这些支架是亲水的,其亲水性和热稳定性随着改性CS的增加而降低,而结晶度则相反。这些支架在促进HepG2细胞增殖及分泌白蛋白和尿素方面表现出良好的性能。此外,与SF/GC或SF/GLC支架相比,SF/LC支架具有更好的肝细胞相容性。To achieve the ideal scaffolds for liver tissue regeneration,chitosan(CS)was modified with lactobionic acid(LA)or/and glycyrrhetinic acid(GA)to obtain LA-modified CS(LC),GA-modified CS(GC)and GA/LA-modified CS(GLC),and the composite nanofibrous scaffolds composed of silk fibroin(SF)and the above modified CS were fabricated by green electrospinning.Fourier transform infrared(FTIR)spectroscopy,nuclear magnetic resonance(NMR)spectroscopy and X-ray diffraction(XRD)patterns were used to characterize the chemical components and structures.The water contact angle was measured to evaluate the hydrophilicity,and thermal gravimetric analysis(TGA)was carried out to obtain the thermal properties.These scaffolds were hydrophilic,and their hydrophilicity and thermal stability decreased with the increase of the modified CS content,while their crystallinity increased.The scaffolds showed good performance in promoting the proliferation of the human hepatoma cell line(HepG2 cell)as well as their secretion of both albumin and urea.Furthermore,the scaffolds with LC had a better performance of hepatocellular compatibility than those with GC or GLC.

关 键 词：甘草次酸(GA) 半乳糖 壳聚糖(CS) 丝素蛋白(SF) 肝组织工程 

分 类 号：R9[医药卫生—药学]