β_(3)-肾上腺素受体在犊牦牛肾周脂肪组织中的表达分布  

Expression and Distribution of β_(3)-AR in Perirenal Adipose Tissue of Yak Calves

作　　者：陈付菊[1] 全七十六 范伟峰 徐伟亮 李颖帮 赵宇田 童依然 陈杏娣 CHEN Fuju;QUAN Qishiliu;FAN Weifeng;XU Weiliang;LI Yingbang;ZHAO Yutian;TONG Yiran;CHEN Xingdi(College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,China;The Promotion Center of Yak Breeding of Datong Hui Tu Autonomous County,Xining 810100,China;Agricultural Comprehensive Administrative Law Enforcement Supervision Bureau of Qinghai Province,Xining 810006,China)

机构地区：[1]青海大学农牧学院,西宁810016 [2]大通回族土族自治县养殖技术推广中心,西宁810010 [3]青海省农业综合行政执法监督局,西宁810006

出　　处：《四川农业大学学报》2024年第3期652-657,共6页Journal of Sichuan Agricultural University

基　　金：青海省科技厅应用基础研究项目(2024-ZJ-785);国家自然科学基金(31960686)。

摘　　要：【目的】探究β_(3)-肾上腺素受体(β_(3)-AR, ADRB3)在犊牦牛寒冷适应中的调控作用。【方法】选取不同日龄(1、7和30日龄)的健康雄性犊牦牛,采用免疫组织化学染色(IHC)、实时荧光定量PCR(qRT-PCR)以及Western blot(WB)对犊牦牛棕色/白色脂肪组织中ADRB3 mRNA及β_(3)-AR蛋白的表达、分布及激素敏感性脂肪酶(HSL)基因mRNA表达进行分析。【结果】IHC结果显示,β_(3)-AR在犊牦牛肾周棕色和白色脂肪细胞中均有分布,且从1~30日龄,β_(3)-AR阳性表达强度显著增加(P<0.05)。qRT-PCR结果显示,从1~30日龄,ADRB3和HSL mRNA表达水平显著增加(P<0.05)。WB结果显示,从1~30日龄,β_(3)-AR蛋白表达水平显著增加(P<0.05)。【结论】ADRB3 mRNA和β_(3)-AR蛋白在犊牦牛肾周棕色和白色脂肪组织中均有表达,且其表达量在白色脂肪组织中高于棕色脂肪组织,说明β_(3)-AR在犊牦牛肾周脂肪组织产热和脂解中均发挥重要的调节作用。【Objective】The aim of this study was to explore the regulatory role ofβ_(3)-adrenergic receptor(β_(3)-AR,ADRB3)in cold adaptation of calf yaks.【Method】Healthy male calf yaks of different ages(1,7 and 30 days)were selected.The expression and distribution of ADRB3 mRNA,β_(3)-AR protein and the hormone-sensitive triglyceride lipase(HSL)mRNA in the perirenal brown and white adipose tissue of calf yaks were detected by immunohistochemical staining(IHC),Real-time fluorescence quantitative PCR(qRT-PCR),and Western blot(WB).【Result】The IHC results showed that theβ_(3)-AR was dis⁃tributed both in perirenal brown and white adipocytes of yak calves,and the positive expression intensity ofβ_(3)-AR was significantly increased from day 1 to 30(P<0.05).The results of qRT-PCR showed that the mRNA expression level of ADRB3 and HSL were significantly increased from day 1 to 30(P<0.05).The re⁃sults of WB showed that the protein expression level ofβ_(3)-AR were significantly increased from day 1 to 30(P<0.05).【Conclusion】ADRB3 mRNA andβ_(3)-AR protein were expressed in both perirenal brown and white adipocytes of yak calves,and its expression level in white adipose tissue were higher than that in brown adipose tissue,indicating thatβ_(3)-AR plays an important regulatory role in nonshivering thermogen⁃esis(NST)and lipolysis in perirenal adipose tissue of yak calves.

关 键 词：犊牦牛 肾周脂肪组织 棕色脂肪组织 白色脂肪组织 β_(3)-肾上腺素受体 

分 类 号：S823.85[农业科学—畜牧学]