一种双金属基磁共振成像纳米探针的构建及其成像性能  

Study on constructing a bimetallic-based magnetic resonance imaging nanoprobes and its contrast ability

作　　者：曹书闻 肖建敏 张桂龙 田梗 魏鹏飞[1,2] CAO Shuwen;XIAO Jianmin;ZHANG Guilong;TIAN Geng;WEI Pengfei(School of Pharmacy,Binzhou Medical University,Yantai 264003,Shandong,P.R.China;Institute of Medical Imaging Probe,Binzhou Medical University,Yantai 264003,Shandong,P.R.China)

机构地区：[1]滨州医学院药学院,山东烟台264003 [2]滨州医学院医学影像探针研究所,山东烟台264003

出　　处：《滨州医学院学报》2024年第3期161-167,共7页Journal of Binzhou Medical University

基　　金：国家自然科学基金青年基金(22007006);山东省泰山学者青年专家(tsqn201909144);山东省自然科学基金青年基金(ZR2022QB133)。

摘　　要：目的通过钒离子嵌入的策略制备一种新型钆钒基纳米颗粒(GdVN),探索其作为T1磁共振成像纳米探针的应用前景。此外,通过调控前驱体的投料比,优化GdVN纳米探针的造影性能,并进一步评估其生物安全性。方法首先,借助透射电子显微镜(TEM)与动态光散射仪(DLS)分别测定GdVN纳米探针的形貌与粒径;随后,通过X射线衍射仪(XRD)、傅里叶变换红外光谱仪(FT-IR)以及X射线光电子能谱(XPS)明确GdVN的晶体结构与组成。其次,利用0.5 T磁共振扫描仪测定纳米探针的T1弛豫率值,筛选出高弛豫率的GdVN。最后,通过MTT细胞实验评估纳米探针的细胞生物相容性。结果成功制备出GdVN纳米探针,并通过调控前驱体的投料比,优化了其造影性能。结果显示,当乙酰丙酮钆与乙酰丙酮氧钒的投料比为1∶4时,所制备的GdVN-2展现出规则的梭形形貌与良好的分散性,粒径大约为90 nm。此外,GdVN-2展示出高T1弛豫率(r1=17.76 mM^(-1) s^(-1)),且细胞实验结果证明GdVN-2具有良好的生物安全性,有利于其作为T1磁共振成像纳米探针在体内的应用。结论本研究所研发的GdVN-2磁共振成像纳米探针展示出优异的T1磁共振造影性能和生物安全性,为实现肿瘤的早期精准诊断提供技术支持。Objective To synthesize a novel gadolinium-vanadium-based nanoparticles(GdVN)as T1 magnetic resonance imaging nanoprobes by using the vanadium ion-doped method,and explore its application as a T1 magnetic resonance imaging nanoprobe;to optimize the contrast ability of GdVN via adjusting the feed ratio of precursors,and evaluate the bio-safety of GdVN.Methods Firstly,the morphology and size of GdVN were measured via the transmission electron microscopy(TEM)and the dynamic light scattering(DLS)detector;Subsequently,the structure and composition of GdVN were analyzed with the X-ray diffraction(XRD),Fourier transform infrared spectroscopy(FT-IR),and X-ray photoelectron spectrometry(XPS),respectively.Secondly,the T1 relaxivity of GdVN was measured with a 0.5 T magnetic resonance imaging scanner to screen out the GdVN with high T1 relaxivity.Finally,the MTT experiments were performed for evaluating the biological safety of nanoprobes.Results GdVN nanoprobes had been successfully prepared by using vanadium ion-doped technology.In addition,the contrast ability of GdVNs had been optimized via adjusting the feed ratio of precursors.When the feeding ratio of gadolinium acetylacetonate to vanadium acetylacetonate was up to 1∶4,the obtained GdVN-2 exhibited regular fusiformis shaped and excellent dispersibility,and the size was approximately 90 nm.Meanwhile,GdVN-2 nanoprobes exhibited the high T1 relaxivity(r1=17.76 mM^(-1) s^(-1)),and the cytotoxicity assay demonstrated that GdVN-2 nanoprobe possessed the excellent bio-safety,which was beneficial to the excellent potential of GdVN-2 as T1 magnetic resonance imaging nanoprobes in vivo.Conclusion In this study,GdVN-2 nanoprobes with uniform morphology exhibits the excellent contrast ability and biosafety,providing the technical support for achieving early and accurate diagnosis of tumors.

关 键 词：钆钒基纳米颗粒 磁共振成像纳米探针 生物安全性 精准诊断 

分 类 号：R811.1[医药卫生—放射医学]