生防芽孢杆菌种子处理剂WS20的研制及对甘蓝枯萎病的防治效果  

出　　处：《农药学学报》2024年第3期426-426,共1页Chinese Journal of Pesticide Science

摘　　要：芽孢杆菌类微生物制剂能有效控制植物病害。本研究在明确生防菌萎缩芽孢杆菌ZY1825菌株对甘蓝枯萎病菌的拮抗抑菌效果及生防菌最佳发酵条件的基础上,以ZY1825菌株发酵液为种子处理剂活性成分,筛选确定了分散剂、成膜剂、稳定剂及防冻剂等助剂组合的最佳质量分数为:木质素磺酸钠4%、羧甲基纤维素0.25%、膨润土1%、乙二醇0.5%,将ZY1825菌株发酵液(9.28×109 cfu/mL)与混合助剂按体积比2:1均质混合,制得生防芽孢杆菌种子处理剂WS20,经测试其成膜时间为1.45 min,包衣均匀度93.06%,货架期可达150 d,热贮和低温稳定性等指标均符合种子处理悬浮剂及微生物制剂的国家相关标准。采用WS20种子处理剂按药种质量比1:30包衣甘蓝种子,对甘蓝枯萎病的盆栽防效为64.94%。研究结果可为利用芽孢杆菌制备种子处理剂以及土传性病害的生物防治提供参考。Bacillus-based microbial agents can effectively control plant diseases.In this study,the antagonistic and inhibitory effects of B.atrophicus ZY1825 on Fusarium wilt of cabbage and the optimal fermentation conditions were revealed.Moreover,using ZY1825 as the active ingredient of the seed treatment agent,the optimum mass fractions of the dispersant,film-forming agent,stabilizer,and anti-freezing agent were selected as sodium lignosulfonate 4%,carboxymethyl cellulose 0.25%,bentonite 1%,and ethylene glycol 0.5%.The biocontrol agent WS20 was prepared by homogenizing the fermentation broth(9.28×109 cfu/mL)of B.astrophile ZY1825 with the mixed adjuvants at a volume ratio of 2:1.The film-forming time of WS20 was 1.45 min,the coating uniformity was 93.06%and its shelf life was up to 150 days.The stability of heat storage and low temperature followed the national standards of seed treatment suspension agents and microbial formulations.The control effect on cabbage wilt was 64.94%when the cabbage seeds were coated with WS20 at a mass ratio of 1:30.The results can provide a reference for the preparation of seed treatment agents and biological control of soil-borne diseases by Bacillus.

关 键 词：种子处理剂 微生物制剂 甘蓝枯萎病 植物病害 生物防治 木质素磺酸钠 低温稳定性 羧甲基纤维素 

分 类 号：S436.35[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]