设施芹菜噻虫嗪和高效氯氟氰菊酯连续施用后消散、积累与风险评估  

Dissipation,accumulation and risk assessment of thiamethoxam and beta-cyfluthrin in greenhouse celery after continuous spraying

作　　者：黄艳萍 王新全[2] 堵紫妍 楚瀚 樊华 韩吉昌 汪志威[2] HUANG Yanping;WANG Xinquan;DU Ziyan;CHU Han;FAN Hua;HAN Jichang;WANG Zhiwei(College of Food and Pharmaceutical Sciences,Ningbo University,Ningbo 315201,China;Institute of Agro-product Safety and Nutrition,Zhejiang Academy of Agricultural Sciences,Hangzhou 310022,China)

机构地区：[1]宁波大学食品与药学学院,宁波315201 [2]浙江省农业科学院农产品质量安全与营养研究所,杭州310022

出　　处：《农药学学报》2024年第3期604-611,共8页Chinese Journal of Pesticide Science

基　　金：浙江省农业农村厅农产品质量安全隐患排查项目(CTZB-2022030457)。

摘　　要：噻虫嗪和高效氯氟氰菊酯是设施芹菜生产中常用的杀蚜虫剂,揭示其消解和积累特征对于评估其膳食摄入风险、优化施用方式具有重要意义。本研究采用气相色谱-串联三重四极杆质谱法,在质谱多反应监测模式下,通过田间残留试验,对2种杀虫剂在3种连续施药方式(组合I:噻虫嗪+高效氯氟氰菊酯,交替II:噻虫嗪-高效氯氟氰菊酯-噻虫嗪,交替III:高效氯氟氰菊酯-噻虫嗪-高效氯氟氰菊酯)下的残留消解动态进行了分析,并对长期膳食摄入风险进行了评估。结果表明:3种连续施药方式下,均在第2次施药(定植后22 d)后原始沉积量最高,首次施药后原始沉积量最低,而芹菜叶面积指数可能是导致该现象的重要因素。根据一级动力学方程拟合获得噻虫嗪和高效氯氟氰菊酯的消解半衰期分别为2.95~3.63 d和2.33~3.11 d。连续3次施药达到安全间隔期(PHI)(7 d)后,噻虫嗪和高效氯氟氰菊酯在组合I中的残留量仅在首次施药后低于MRL (噻虫嗪:1 mg/kg;高效氯氟氰菊酯:0.5 mg/kg);而交替II中2种农药残留量在3次施药后均低于MRL;交替III中高效氯氟氰菊酯残留量在第3次施药后超过了MRL。鉴于噻虫嗪施药后可代谢为噻虫胺,本研究亦对噻虫胺残留量进行了监测,发现其在施药后3~5 d达到最大值,但在达到安全间隔期后,噻虫胺的残留量均未超过其MRL (0.04mg/kg),表明由噻虫嗪代谢导致噻虫胺残留超标的风险较低。膳食摄入风险评估结果显示:在本次于浙江绍兴进行的田间规范残留试验中,噻虫嗪和高效氯氟氰菊酯组合和交替连续施药3次,安全间隔期7 d后,所采集的芹菜样品中噻虫嗪和高效氯氟氰菊酯残留对风险商的贡献率(0.11%~3.76%)低于100%,其长期膳食摄入风险均处于可接受水平。研究表明,相较于组合施药,交替用药可在一定程度减少农药残留,而噻虫嗪-高效氯氟氰菊酯-噻虫嗪交替施用表现出最低的残Thiamethoxam and beta-cyfluthrin are commonly used insecticides for aphid control in greenhouse celery.It is of great significance to reveal their dissipation and accumulation characteristics for evaluating their dietary intake risks and optimizing application methods.Based on this,gas chromatography-tandem triple quadrupole mass spectrometry was used in the multi-reaction monitoring mode of mass spectrometry,The dynamics of residue digestion of two insecticides under three repeated spraying methods(combination I:thiamethoxam+beta-cyfluthrin,alternating II:thiamethoxam-betacyfluthrin-thiamethoxam,alternating III:beta-cyfluthrin-thiamethoxam-beta-cyfluthrin)and assessed the risk of chronic dietary intake.The results showed under the three continuous spraying methods,the original deposition amount was the highest after the second spraying(22 d after planting)and the lowest after the first spraying;and the celery leaf area index may be an important factor causing this phenomenon.According to the first-order kinetic equations,the digestion half-life of thiamethoxam and beta-cyfluthrin were 2.95-3.63 d and 2.33-3.11 d,respectively.Comparative analysis of residues after the safety interval of 7 d after three repeated spraying,residue of thiamethoxam and beta-cyfluthrin in combination I was higher than maximum residue limit(MRL)except for the first spraying(thiamethoxam:1mg/kg;beta-cyfluthrin:0.5 mg/kg);However,the residue of beta-cyfluthrin in alternating III exceeds the MRL after the last spraying.Since thiamethoxam could be metabolized to clothianidin,dynamic residue of clothianidin was determined.The highest concentration was obtained at 3-5 d after spraying.However,the residue of clothianidin was lower than MRL(0.04 mg/kg)after the pre-harvest interval(PHI);The results of risk assessment of dietary intake showed that in this standardized residue test in Shaoxing,Zhejiang Province,thiamethoxam and beta-cyfluthrin were combined and applied alternatly for three times.After the PHI of 7 d,the contribution rate of thiam

关 键 词：噻虫嗪 高效氯氟氰菊酯 噻虫胺 设施农业 芹菜 残留 膳食风险评估 

分 类 号：TQ450.26[化学工程—农药化工] S482.3[农业科学—农药学]