丙酸盐对厌氧氨氧化生物膜系统的影响及机制研究  

Effect and Mechanism of Propionate on ANAMMOX Biofilm Systems

作　　者：朱如龙 刘佐丞 王永才 熊辉 史济民 赵伟华 苗蕾 Zhu Rulong;Liu Zuocheng;Wang Yongcai;Xiong Hui;Shi Jimin;Zhao Weihua;Miao Lei(Power China Huadong Engineering Corporation Limited,Hangzhou 311122,China;School of Environmental Science and Engineering,Huazhong University of Science and Technology,Wuhan 430074,China;School of Environmental and Municipal Engineering,Qingdao University of Technology,Qingdao 266033,China)

机构地区：[1]中国电建集团华东勘测设计研究院有限公司,浙江杭州311122 [2]华中科技大学环境科学与工程学院,湖北武汉430074 [3]青岛理工大学环境与市政工程学院,山东青岛266033

出　　处：《市政技术》2024年第6期227-236,共10页Journal of Municipal Technology

摘　　要：厌氧氨氧化因具有能耗低、经济效益好等优势,在污水脱氮中具有较好的应用前景,然而在处理实际污水时其易受有机物影响,且不同种类的有机物对厌氧氨氧化也会产生不同影响。该研究发现,当厌氧氨氧化生物膜系统中存在低质量浓度丙酸盐(30 mg/L)时,丙酸盐优先参与到硝态氮的短程反硝化过程,厌氧氨氧化活性受到轻微抑制(反应速率从7.24 mg/(L·h)降至5.56 mg/(L·h))。当系统中丙酸盐质量浓度提高到60 mg/L时,其影响随着系统初始硝态氮质量浓度的变化而变化。当系统初始硝态氮质量浓度较低时(<15 mg/L),丙酸盐除了参与硝态氮的短程反硝化外,还有部分参与到亚硝态氮的反硝化过程,同时进一步抑制厌氧氨氧化的活性;当初始硝态氮质量浓度较高(>30 mg/L)时,丙酸盐又只参与到硝态氮的短程反硝化过程。根据丙酸盐质量浓度调整进水基质浓度配比,系统总氮去除率可达到94%。宏基因检测结果表明,Candidatus_Kuenenia和Candidatus_Brocadia是系统中主要的厌氧氨氧化菌属。随着丙酸盐质量浓度的增加,Candidatus_Kuenenia和Candidatus_Brocadia的相对丰度从8.83%和1.78%降至2.88%和0.20%,而反硝化菌属Thauera的相对丰度从最初的0上升到19.39%。但当系统处在进水基质最佳浓度配比时,Candidatus_Kuenenia和Candidatus_Brocadia的相对丰度再次出现小幅度上升(6.76%和0.30%),而Thauera的相对丰度则下降到14.82%。该研究将有助于为厌氧氨氧化工艺在污水处理中的实际应用和优化运行提供理论指导。Anaerobic ammonium oxidation(ANAMMOX)process has a good application prospect in nitrogen removal from wastewater due to its low energy consumption and high cost-effectiveness.However,it could be affected by different types of organic matters when treating the actual wastewater.In this work,when the low concentration of propionate(30 mg/L)was added into the ANAMMOX biofilm system,the propionate was prior to participate in the partial denitrification process,and ANAMMOX activity was inhibited slightly,with the reaction rate decreasing from 7.24 mg/(L·h)to 5.56 mg/(L·h).When the concentration of propionate in the system increased to 60 mg/L,the performance changed with the initial nitrate concentration in the system.Under the low concentration of initial nitrate(<15 mg/L),the propionate involved in the partial denitrification via nitrate,and the whole denitrification via nitrite;the activity of ANAMMOX bacteria was further inhibited.Under the high initial nitrate concentration(above 30 mg/L),the propionate mainly involved in the partial denitrification via nitrate.Based on the effect of propionate,the influent concentration of this ANAMMOX system was optimized,leading to the total nitrogen removal efficiencyreaching about 94%.Candidatus_Kuenenia and Candidatus_Brocadia were identified as the dominant ANAMMOX bacteria.With the increasement of propionate concentration,the relative abundance of Candidatus_Kuenenia and Candidatus_Brocadia decreased from 8.83%and 1.78%to 2.88%and 0.20%,respectively.However,the relative abundance of denitrifying bacteria Thauera increased from 0 to 19.39%.When the system was fed with the optimal influent,the relative abundance of Candidatus_Kuenenia and Candidatus_Brocadia increased slightly to 6.76%and 0.30%,respectively,and the relative abundance of Thauera decreased to 14.82%.This studyaimed to provide theoretical guidance for the practical application and optimization of ANAMMOX process in wastewater treatment.

关 键 词：厌氧氨氧化 有机物 丙酸盐 微生物群落 宏基因组 

分 类 号：X703[环境科学与工程—环境工程]