大鼠肩袖撕裂重建过程中色氨酸代谢的变化  

Tryptophan metabolism alters in rotator cuff tear repair in a rat model

作　　者：朱东旭 黄晓红 朱馨蕊 于腾波 张英泽[4,6] Zhu Dongxu;Huang Xiaohong;Zhu Xinrui;Yu Tengbo;Zhang Yingze(Department of Medicine,Qingdao University,Qingdao 266000,China;Department of Sports Medicine,The Affiliated Hospital of Qingdao University,Qingdao 266100,China;Shandong Institute of Traumatic Orthopedics,Medical Research Center,The Affiliated Hospital of Qingdao University,Qingdao 266000,China;Department of Orthopedics,The Third Hospital of Hebei Medical University,Shijiazhuang 050051,China;Department of Orthopedic Surgery,Qingdao Municipal Hospital,Qingdao 266071,China;Department of Orthopedics,The Affiliated Hospital of Qingdao University,Qingdao 266000,China)

机构地区：[1]青岛大学医学部,青岛266000 [2]青岛大学附属医院运动医学科,青岛266100 [3]青岛大学附属医院医学研究中心山东省创伤骨科研究所,青岛266100 [4]河北医科大学第三医院骨科医院,石家庄050051 [5]青岛市市立医院骨外科,青岛266071 [6]青岛大学附属医院骨科,青岛266100

出　　处：《中华创伤骨科杂志》2024年第5期435-443,共9页Chinese Journal of Orthopaedic Trauma

摘　　要：目的比较骨重塑能力正常和骨重塑能力缺陷大鼠肩袖撕裂(RCT)修复术后色氨酸代谢的差异,探讨色氨酸代谢是否与骨长入有关。方法共选取50只成年雌性Sprague-Dawley(SD)大鼠进行研究,随机选择18只大鼠接受双侧卵巢摘除手术(OVX),设为OVX组;余32只大鼠接受双侧卵巢摘除假手术,设为假手术组。术后3个月,OVX组和假手术组各自随机选择4只大鼠,采用显微计算机断层扫描(Micro-CT)鉴定肱骨头的骨量流失情况。OVX组剩余大鼠进行RCT造模,并接受急性冈上肌横断修复术,设为OVX+RCT组(n=14);假手术组剩余大鼠分别选取14只接受急性冈上肌横断修复术和RCT假手术,分别设为RCT组(n=14)和SO组(n=14)。修复手术后2周检测大鼠抓力,并取出冈上肌腱骨复合体,通过实时荧光定量多聚酶链式反应(qPCR)检测、苏木素伊红(HE)染色和免疫组化(IHC)染色评估新骨生成和骨长入情况,采用超高效液相色谱(UPLC)分析检测色氨酸代谢。结果抓力测试结果表明,SO组大鼠抓力最大,RCT组次之,OVX+RCT组抓力最小,三组间差异有统计学意义(P<0.05)。qPCR分析结果表明,SO组腱骨复合体的骨硬化素相对表达量显著高于OVX+RCT组和RCT组,IHC染色显示RCT组的骨织素相对表达量显著高于SO组和OVX+RCT组,以上项目组间差异均有统计学意义(P<0.05)。UPLC检测结果表明,SO组、RCT组、OVX+RCT组肩袖腱骨复合体色氨酸含量相近,差异无统计学意义(P>0.05)。qPCR结果表明,犬尿酸代谢途径的吲哚胺2,3-双加氧酶2的表达量在SO组、RCT组、OVX+RCT组呈递增趋势:OVX+RCT组显著高于SO组,OVX+RCT组的3-羟基邻氨基苯甲酸3,4-双加氧酶的表达量显著高于RCT组和SO组,5-羟色胺代谢途径的单胺氧化酶A亚型和B亚型在SO、RCT、OVX+RCT三组间递增,OVX+RCT组吲哚代谢途径的多巴脱羧酶的表达量显著高于SO组,而细胞色素P450氧化还原酶的表达量在SO、RCT、OVX+RCT三组间递增,以上项Objective To explore whether tryptophan metabolism is related to bone growth by comparing the differences in tryptophan metabolism after rotator cuff tear(RCT)repair between rats with normal bone remodeling capability and those with defective bone remodeling capability.Methods Of the 50 adult female Sprague Dawley(SD)rats used for this study,18 were randomly selected to undergo bilateral ovariectomy(OVX)(OVX group),and the remaining 32 underwent bilateral OVX sham surgery(sham surgery group).All rats were maintained in standard conditions.Three months after surgery,4 rats were randomly selected from OVX group and the sham surgery group,respectively,for Micro CT identification of bone loss at the rat humeral head.The remaining rats in OVX group underwent RCT modeling and surgical repair of acute supraspinatus muscle transection(OVX+RCT group)(n=14);the remaining rats in the sham surgery group were subjected to either surgical repair of acute supraspinatus muscle transection(RCT group,n=14)or RCT sham surgery(SO group,n=14).The grip power of the rats was tested 2 weeks after surgery.The supraspinatus tendon-bone complex was harvested for evaluation of new bone formation and growth through real-time fluorescence quantitative polymerase chain reaction(qPCR),hematoxylin eosin(HE)staining,and immunohistochemistry(IHC)staining.The tryptophan metabolism was analyzed using ultra high performance liquid chromatography(UPLC).Results The grip power test showed that SO group had the greatest grip power,followed by RCT group and OVX+RCT group,with statistically significant differences between the 3 groups(P<0.05).The qPCR showed that the relative expression of osteosclerosis in the tendon-bone complex in SO group was significantly higher than that in OVX+RCT group and RCT group;IHC staining showed that the relative expression of osteocalcin in RCT group was significantly higher than that in SO group and OVX+RCT group(P<0.05).The UPLC showed that the content of tryptophan in the rotator cuff tendon-bone complex was similar amon

关 键 词：色氨酸 撕裂伤 骨质疏松 肩袖撕裂 骨插入 

分 类 号：R687.4[医药卫生—骨科学]