FGF13通过ROS介导抑制乳腺癌细胞MCF7凋亡  

FGF13 inhibits apoptosis through regulating intracellular ROS in MCF7 cells

作　　者：莫莎 唐铖铖 刘怡 路宏朝[1] 马海东 曾文先 MO Sha;TANG Chengcheng;LIU Yi;LU Hongzhao;MA Haidong;ZENG Wenxian(School of Biologic Science and Engineering,Shaanxi University of Technology,Hanzhong 723000;School of Medical Technology,Hanzhong Vocational and Technical College,Hanzhong 723000,China)

机构地区：[1]陕西理工大学生物科学与工程学院,陕西汉中723000 [2]汉中职业技术学院医学技术学院,陕西汉中723000

出　　处：《生物技术》2024年第2期210-216,236,共8页Biotechnology

基　　金：陕西省教育厅专项科研计划项目(20JK0570)。

摘　　要：[目的]探究成纤维细胞生长因子13(Fibroblast growth factor 13,FGF13)对乳腺癌细胞MCF7凋亡的调控作用。[方法]Western Blotting和免疫组织化学染色检测FGF13的表达;构建干扰和超表达FGF13的MCF7细胞株;TUNEL法和Western Blotting检测细胞凋亡;DCFH-DA荧光探针法检测细胞内活性氧(Reactive oxygen species, ROS)。[结果]与正常乳腺细胞相比,FGF13在乳腺癌MCF7细胞中表达升高(0.16±0.01 vs 3.89±0.23,P<0.001)。干扰FGF13后,MCF7细胞凋亡率升高(2.20%±0.10%vs 10.79%±0.69%,P<0.001),促凋亡蛋白Bax(0.99±0.02 vs 2.56±0.02)、Caspase 3(1.00±0.01 vs 1.70±0.01)和p53(0.98±0.01 vs 2.22±0.03)的表达上调(P<0.001),而抗凋亡蛋白Bcl-xl(0.99±0.01 vs 0.29±0.01)、Bcl-2(0.99±0.01 vs 0.27±0.01)的表达下调(P<0.001)。相反,超表达FGF13后,细胞凋亡率和促凋亡蛋白的表达均降低(P<0.001),而抗凋亡蛋白的表达均升高(P<0.001)。而且,干扰FGF13的表达引起细胞内ROS水平升高(1 552.00±51.37 vs 2 714.00±55.19,P<0.001),而超表达则导致ROS减少(2 102.83±65.29 vs 1 111.67±63.53,P<0.001)。[结论]FGF13可能通过ROS介导抑制乳腺癌MCF7细胞凋亡。[Objective]To investigate the effect of fibroblast growth factor 13(FGF13)on the regulation of apoptosis in breast cancer MCF7 cell line.[Method] Western Blotting and immunohistochemical staining were used to detect FGF13 expression.The MCF7 cell lines with stable interference and over-expression of FGFI3 were constructed.TUNEL and western blot were applied to detect apoptosis.DCFH-DA fluorescence probe was used to measure cellular reactive oxygen species(ROS).[Result]Compared with the health breast cells,FCF13 expression increased in MCF7 cells(0.16±0.01 us 3.89±0.23,P<0.001).Knockdown of FGF13 expression resulted in increase(P<0.001)of apoptosis(2.20%±0.10% vs 10.79%±0.69%)and expression of pro-apoptotic proteins Bax(0.99±0.02 us 2.56±0.02),Caspase 3(1.00±0.01 vs 1.70±0.01)and p53(0.98±0.01 us 2.22±0.03),and decrease(P<0.001)of anti-apoptotic preoteins Bcl-xl(0.99±0.01 us 0.29±0.01)and Bcl-2(0.99±0.01 vs 0.27±0.01).On the contrary,over-expression of FGF13 led to decrease of apoptosis and expression of the pro-apoptotic proteins(P<0.001),and increase of expression of the anti-apoptotic pretoeins(P<0.001).Furthermore,knockdown of FGF13 expression yielded accumulation of ROS(1552.00±51.37 vs 2714.00±55.19,P<0.001),while FCF13 overexpression led to decrease of R0S(2102.83±65.29 us 1111.67±63.53,P<0.001).[Conclusion]FGF13 perhaps inhibits apoptosis through regulating intracellular ROS in MCF7 cells.

关 键 词：乳腺癌 细胞凋亡 成纤维细胞生长因子13 活性氧 抗凋亡蛋白 促凋亡蛋白 基因表达 半胱天冬酶 

分 类 号：Q255[生物学—细胞生物学]