3.0 T谷氨酸化学交换饱和转移评估肾脏缺血再灌注损伤氧化还原代谢的实验研究  

Experimental study on evaluating renal redox metabolism in renal ischemia-reperfusion injury using GluCEST imaging with 3.0 T MRI

作　　者：米文夏 邢兆宇[2] 潘靓[1] 虞心田 陈杰[1] 邢伟[1] Mi Wenxia;Xing Zhaoyu;Pan Liang;Yu Xintian;Chen Jie;Xing Wei(Department of Radiology,First People′s Hospital of Changzhou,Changzhou 213003,China;Department of Urology,First People′s Hospital of Changzhou,Changzhou 213003,China)

机构地区：[1]常州市第一人民医院放射科,常州213003 [2]常州市第一人民医院泌尿外科,常州213003

出　　处：《中华内科杂志》2024年第6期593-599,共7页Chinese Journal of Internal Medicine

基　　金：国家自然科学基金(82001763,82171901,82302141)。

摘　　要：目的探讨3.0 T谷氨酸化学交换饱和转移(GluCEST)成像评估肾脏缺血再灌注损伤(IRI)氧化还原代谢的可行性。方法IRI组实验兔(n=56)夹闭左肾动脉45 min后松开建立肾IRI模型,于术前、术后1 h、12 h、1 d、3 d、7 d、14 d行GluCEST MRI检查,每次扫描后处死8只IRI组实验兔,假手术组实验兔(n=8)于术后14 d扫描后处死,行肾脏病理和活性氧(ROS)检测。比较各组肾脏皮质和外髓非对称磁化传递率(MTR_(asym))的差异,分析肾脏MTR_(asym)与ROS水平的相关性。结果假手术组与IRI各亚组肾脏皮质MTR_(asym)均高于外髓(t=8.16,P<0.001;t=4.78,P=0.002;t=4.94,P=0.002;t=5.76,P=0.001;t=6.68,P<0.001;t=6.40,P<0.001;t=5.16,P=0.001;t=3.30,P=0.013)。IRI-1h、IRI-12h、IRI-1d、IRI-3d、IRI-7d及IRI-14d组皮质和外髓MTR_(asym)均低于假手术组和IRI-pre组。IRI-1h组皮质和外髓MTR_(asym)均低于IRI-12h、IRI-1d、IRI-3d、IRI-7d及IRI-14d组(均P<0.05)。IRI-12h组皮质MTR_(asym)显著低于IRI-7d、IRI-14d组(1.84%±0.09%比2.42%±0.19%和2.41%±0.31%,均P<0.05),IRI-1d组皮质MTR_(asym)显著低于IRI-7d组(1.99%±0.17%比2.42%±0.19%,P=0.008)。IRI-12h组外髓MTR_(asym)显著低于IRI-3d、IRI-7d、IRI-14d组(1.32%±0.27%比1.79%±0.31%,1.98%±0.18%,1.66%±0.40%,均P<0.05),IRI-7d组外髓MTR_(asym)显著高于IRI-1d、IRI-14d组(1.98%±0.18%比1.52%±0.31%和1.66%±0.40%,均P<0.05)。肾脏皮质和外髓MTR_(asym)与ROS平均荧光强度呈强负相关(ρ=-0.889,P<0.001;ρ=-0.784,P<0.001)。结论3.0 T GluCEST可间接反映肾脏IRI氧化还原代谢的变化。Objective To investigate the feasibility of 3.0 T glutamate chemical exchange saturation transfer(GluCEST)imaging in evaluating renal redox metabolism in renal ischemia-reperfusion injury(IRI).Methods Rabbits in the IRI group(n=56)underwent surgery by clamping the left renal artery for 45 min and then releasing to establish IRI.Rabbits in the sham group(n=8)underwent the same operation without clamping the left renal artery.GluCEST MRI was performed before and at 1 h,12 h,1 day,3 days,7 days,and 14 days after the operations,with eight rabbits in the IRI group sacrificed immediately after each scanning and eight in the sham group sacrificed at 14 days after scanning.The left kidneys were removed for histopathological examination and reactive oxygen species(ROS)fluorescence staining.Differences in the magnetic resonance ratio asymmetry(MTR_(asym))of the renal cortex and outer medulla among different groups were compared.Correlations between the MTR_(asym)and ROS were analyzed.Results The MTR_(asym)of the renal cortex in the sham and IRI subgroups were higher than that of the outer medulla(t=8.16,P<0.001;t=4.78,P=0.002;t=4.94,P=0.002;t=5.76,P=0.001,t=6.68,P<0.001;t=6.40,P<0.001;t=5.16,P=0.001;t=3.30,P=0.013).The MTR_(asym)of the renal cortex and outer medulla in the IRI-1h,IRI-12h,IRI-1d,IRI-3d,IRI-7d,and IRI-14d groups were lower than in the sham and IRI-pre groups(all P<0.05).The MTR_(asym)of the renal cortex and outer medulla in the IRI-1h group were lower than in the IRI-12h,IRI-1d,IRI-3d,IRI-7d,and IRI-14d groups(all P<0.05).The MTR_(asym)of the renal cortex in the IRI-12h group was lower than in the IRI-7d and IRI-14d groups(1.84%±0.09%vs.2.42%±0.19%,2.41%±0.31%,all P<0.05).The MTR_(asym)of the renal cortex in the IRI-1d group was lower than in the IRI-7d group(1.99%±0.17%vs.2.42%±0.19%,P=0.008).The MTR_(asym)of the outer medulla in the IRI-12h group was lower than in the IRI-3d,IRI-7d,and IRI-14d groups(1.32%±0.27%vs.1.79%±0.31%,1.98%±0.18%,1.66%±0.40%,respectively,all P<0.05).The MTR_(asym)of the ou

关 键 词：磁共振成像 肾 缺血 再灌注损伤 氧化性应激 

分 类 号：R692[医药卫生—泌尿科学]