HPLC同时测定沙棘中的6种色胺类生物碱  

Simultaneous determination of six tryptamine alkaloids in Hippophae rhamnoides by HPLC

作　　者：刘振华 任李成城 王月 董琦[2] 王洪伦[2] 胡娜[2] LIU Zhenhua;REN Lichengcheng;WANG Yue;DONG Qi;WANG Honglun;HU Na(School of Medicine,Qinghai University,Xining,Qinghai,810001 P.R.China;Key Laboratory of Tibetan Medicine Research,Qinghai Provincial Key Laboratory of Tibetan Medicine Research,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining,Qinghai,810001 P.R.China)

机构地区：[1]青海大学医学院,青海西宁810001 [2]中国科学院西北高原生物研究所中国科学院藏药研究重点实验室青海省藏药研究重点实验室,青海西宁810008

出　　处：《华西药学杂志》2024年第3期321-323,共3页West China Journal of Pharmaceutical Sciences

基　　金：中国科学院王宽诚率先人才计划“产研人才扶持项目”(2021);青海省创新平台建设专项(2021-ZJ-T05)。

摘　　要：目的采用HPLC法同时测定6种沙棘中的色胺类生物碱。方法采用Xcharge-C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-0.15%三氟乙酸水溶液,梯度洗脱,流速1.0 mL·min^(-1),检测波长280 nm,柱温30℃,进样量10μL。结果6种色胺类生物碱的线性范围分别为6.28~251.40、5.71~228.60、1.14~457.10、0.80~32.00、0.31~12.60、0.46~18.30μg·mL^(-1)(r^(2)>0.9993);其中,供试品中共检测到4种色胺类生物碱,分别是5-羟色胺、6-hydroxy-l-methyltetrahydro-β-carboline-1-carboxylic acid、shepherdine、L-色氨酸;平均加样回收率分别为93.63%、89.86%、91.58%、90.91%,RSD均<3.00%。结论所用方法简便、可靠、重复性好、结果准确。OBJECTIVE To simultaneous determine the of 6 tryptamine alkaloids in Hippophae rhamnoides L.by HPLC.METHODS The separation was performed on Xcharge-C_(18)(250 mm×4.6 mm,5μm)column with mobile phase consisted of methanol-0.15%trifluoroacetic acid solution by gradient elution at the flow rate of 1.0 mL·min"1.The wavelength was set at 280 nm,column temperature was 30℃and the injection volume was 10μL.RESULTS The linear ranges of 6 tryptamine alkaloids were 6.28-251.40μgmL-1,5.71-228.60μg·mL^(-1),1.14-457.10μg·mL^(-1),0.80-32.00μg·mL^(-1),0.31-12.60μg·mL^(-1)and 0.46-18.30μg·mL^(-1)(r^(2)>0.9993),respectively.Four kinds of tryptamine alkaloids were detected in the test samples,which were 5-hydroxytryptamine,6-hydroxy-1-methyltetrahydro-β-carboline-1-carboxylic acid,shepherdine and L-tryptophan.The average recoveries were 93.63%,89.86%,91.58%and 90.91%,respectively.The RSDs were less than 3.00%.CONCLUSION The method is simple and reliable,with good repeatability and accurate results.

关 键 词：沙棘 高效液相色谱法 色胺类生物碱 5-羟色胺 L-色氨酸 Shepherdine 含量测定 

分 类 号：R917[医药卫生—药物分析学]