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作 者:赵永树 张世超 杨澜 谢佳鑫 刘明[1] 杨汉波 辜云杰[1] 彭建[1] 刘闵豪 ZHAO Yongshu;ZHANG Shichao;YANG Lan;XIE Jiaxin;LIU Ming;YANG Hanbo;GU Yunjie;PENG Jian;LIU Minhao(Ecological Restoration and Conservation on Forest and Wetland Key Laboratory of Sichuan Province,Sichuan Academy of Forestry,Chengdu 610081,China;Meishan Dongpo District Forestry Resource Service Station,Meishan 620020,China;Sichuan Provincial Key Laboratory of Ecological Forestry Engineering on the Upper Reaches of the Yangtze River,National Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River,Rainy Area of West China Plantation Ecosystem Permanent Scientific Research Base,College of Forestry,Sichuan Agricultural University,Chengdu 611130,China;Sichuan Chaoyuan Landscape Planning and Design Company Co.Ltd.,Chengdu 610084,China)
机构地区:[1]四川省林业科学研究院,森林和湿地生态恢复与保育四川重点实验室,四川成都610081 [2]眉山市东坡区林业资源服务站,四川眉山620020 [3]长江上游林业生态工程四川省重点实验室,长江上游森林资源保育与生态安全国家林业和草原局重点实验室,华西雨屏区人工林生态系统研究长期科研基地,四川农业大学林学院,四川成都611130 [4]四川超远风景园林规划设计有限公司,四川成都610084
出 处:《四川林业科技》2024年第3期1-8,共8页Journal of Sichuan Forestry Science and Technology
基 金:四川省财政专项创新团队项目—桢楠基本群体遗传多样性研究(2023LCTD0203);四川省科技支撑计划项目(2021YFYZ0032);四川省自然科学基金(2022NSFSC1062);世界银行贷款四川省长江经济带珍稀树种保护与发展项目(510201202038467);四川农业大学科研兴趣培养计划项目(2023200)资助。
摘 要:楠木(Phoebe zhennan)是我国特有的珍贵用材树种。利用14对多态性SSR引物对四川省10个种源39个楠木家系进行遗传多样性特征分析,以期揭示其遗传背景及家系间的遗传关系,为楠木优良种源(家系)的选择与利用提供理论依据。结果显示,14对SSR引物共检测出178个等位基因(Na),平均有效等位基因数(Ne)为7.058。PIC值在0.306~0.939间变化,均值为0.746,多态性较高。Shannon’s信息指数(I)在1.306~1.511之间变化,平均值为1.368,表明楠木种源(家系)具有较高的遗传多样性水平。期望杂合度(He)与观测杂合度(Ho)分别在0.646~0.0.703之间和0.920~1.000之间,平均分别为0.676和0.550。分子方差分析(AMOVA)结果显示,99%的遗传变异来源于种源内,仅1%的遗传变异来源于种源间。基于STRUCTURE对39个家系进行群体结构分析,当K=3时,ΔK有最大峰值,表明适宜被分为3个群组。基于遗传距离的聚类分析将楠木39个家系分为4个类群,除F20单独一个类群外,其余三个类群混合了成都、泸州、雅安、乐山各地的楠木家系,类群划分与种源地无明显联系。研究结果为楠木优异基因资源的发掘和杂交亲本的选配提供了理论参考。Pheobe zhennan is a unique and valuable timber tree plants in China.In order to reveal the genetic background and relationship among families,and to provide theoretical basis for the utilization of P.zhennan,14 pairs of polymorphic SSR primers were used to analyze the genetic diversity of 39 P.zhennan families from 10 provenances in Sichuan Province.The results showed that a total of 178 alleles(Na)were detected by 14 pairs of SSR primers,and the average effective allele number(Ne)was 7.058.The PIC value varied from 0.306 to 0.939,with an average value of 0.746,showing high polymorphism of the SSR markers.Shannon's information index(I)ranged from 1.306 to 1.511,with an average value of 1.368,indicating that the P.zhennan provenances(families)had a relative high level of genetic diversity.The expected heterozygosity(He)and the observed heterozygosity(Ho)were 0.646-0.703 and 0.920-1.000 respectively,with an average of 0.676 and 0.550,respectively.The results of molecular analysis of variance(AMOVA)showed that 99%of genetic variation came from within provenances,and only 1%came from among provenances.Based on STRUCTURE software,the genetic structure was analyzed.When K=3,theΔK had the maximum peak value,indicating that it was suitable to be divided into three groups.Based on the cluster analysis of genetic distance,39 P.zhennan families were divided into four groups.Except for a single group of F20,the other three groups were mixed with other P.zhennan families from Chengdu,Luzhou,Ya'an and Leshan,and there was no obvious relationship between the classification of groups and the provenance sites.The study results provided a theoretical reference for the exploration of excellent gene resources and the selection of hybrid parents of P.zhennan.
分 类 号:S792.24[农业科学—林木遗传育种]
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