白及叶枯病病原菌分离鉴定与致病性测定  

Isolation,identification and pathogenicity determination of pathogen of Bletilla Striata leaf blight

作　　者：刘培琴 姜丽琼[1] 卢麒宇 刘继[1] 谢永东 浣杰 李思言 郑雯雪 李文俊[1] LIU Peiqin;JIANG Liqiong;LU Qiyu;LIU Ji;XIE Yongdong;HUAN Jie;LI Siyan;ZHENG Wenxue;Li Wenjun(Chengdu Academy of Agricultural and Forestry Sciences,Chengdu 611130,China)

机构地区：[1]成都市农林科学院,四川成都611130

出　　处：《四川林业科技》2024年第3期142-146,共5页Journal of Sichuan Forestry Science and Technology

基　　金：成都市财政项目“都市现代农业集成示范”(51010023T000008361897);“高质量天府粮仓功能性作物开发与产业化技术集成示范”(51010023T000008363007)。

摘　　要：为明确白及叶枯病病原菌种类,对白及叶枯病病原进行分离、纯化及rDNA-ITS序列分析鉴定其种属,并将纯化病原菌回接于白及植株,评价其致病性。结果表明,从白及患病样品上共分离6株真菌,其中有三株镰刀菌,分别为角化镰刀菌(Fusarium keratoplasticum)、尖孢镰刀菌(Fusarium oxysporum)、木贼镰刀菌(Fusarium equiseti);一株曲霉菌,为日本曲霉(Aspergillus japonicus);一株灰霉,为灰葡萄孢霉(Botrytis cinerea);一株毛霉,为易脆毛霉(Mucor fragilis)。经致病性测定,发现上述6株真菌对白及均有致病性。In order to clarify the pathogenic bacteria species of Bletilla Striata leaf blight,the pathogen of B.Striata leaf blight was isolated,purified and identified by rDNA-ITS sequence analysis,the purified pathogen was grafted to B.Striata plants to evaluate its pathogenicity.The results showed that a total of 6 fungi strains were isolated from the diseased samples of B.striata,including three strains of Fusarium,namely:F.keratoplasticum,F.oxysporum and F.equiseti;a strain of Aspergillus japonicus;a strain of Botrytis cinerea;a strain of Mucor fragilis.According to the pathogenicity test,it was found that the above 6 strains of fungi were all pathogenic to B.Striata.

关 键 词：白及 叶枯病 病原鉴定 致病性 

分 类 号：S435.672[农业科学—农业昆虫与害虫防治]