靶向B7-H3抑制人脐静脉血管内皮细胞生长、迁移和血管形成  

Targeting B7-H3 inhibited growth,migration,and angiogenesis of human umbilical vein endothelial cells

作　　者：刘宇 王南楠 牛可 凌惠娟 朱亚玉 胡伟[2] 陈礼文[3] LIU Yu;WANG Nan-nan;NIU Ke;LING Hui-juan;ZHU Ya-yu;HU Wei;CHEN Li-wen(Dept of Clinical Laboratory,the Second Hospital of Anhui Medical University,Hefei 230601,China;Dept of Clinical Pharmacology,the Second Hospital of Anhui Medical University,Hefei 230601,China;Dept of Blood Transfusion,the Second Hospital of Anhui Medical University,Hefei 230601,China)

机构地区：[1]安徽医科大学第二附属医院检验科,安徽合肥230601 [2]安徽医科大学第二附属医院临床药物实验中心,安徽合肥230601 [3]安徽医科大学第二附属医院输血科,安徽合肥230601

出　　处：《中国药理学通报》2024年第7期1311-1316,共6页Chinese Pharmacological Bulletin

基　　金：安徽省临床医学研究转化项目(No 202304295107020019)。

摘　　要：目的探讨靶向抑制协同信号分子B7-H3对人脐静脉血管内皮细胞(human umbilical vein endothelial cells,HUVECs)生长、迁移及血管生成能力影响。方法使用小干扰RNA(small interfering RNA,siRNA)敲减HUVECs B7-H3分子,采用CCK-8实验检测24 h、48 h和72 h细胞增殖;采用Transwell实验检测24 h细胞迁移;三维细胞培养观察细胞血管生成。结果与空序列转染的阴性对照(siRNA-Control)组比较,siRNA-720、siRNA-1707和siRNA-16903条备选siRNA对B7-H3表达的抑制效果不同,siRNA-1690抑制率明显高于siRNA-720和siRNA-1707,因此,选择使用siRNA-1690序列进行后续实验。CCK-8细胞活力实验结果显示,敲减B7-H3后24 h、48 h和72 h HUVECs增殖能力分别下降24%、22%(P>0.05,与24 h比较)和15%(P<0.05,与48 h比较);Transwell迁移实验表明,与siRNA-Control组对比,敲减B7-H3的HUVECs 24 h迁移能力明显减低(P<0.01);三维细胞培养结果表明,采用si-B7-H3敲减B7-H3基因后,HUVECs血管生成能力明显下降(P<0.01)。结论靶向B7-H3抑制HUVECs生长、迁移及血管生成。Aim To explore the effect of targeted inhibition of co-signaling molecule B7-H3 on the growth,migration,and angiogenesis ability of human umbilical vein endothelial cells(HUVECs).Methods Small interference RNA was used to knock down HUVECs B7-H3 molecules.CCK-8 test was used to detect cell proliferation at 24 h,48 h and 72 h.Transwell test was then used to detect 24 h cell migration,and three-dimensional cell culture was used to observe cell angiogenesis.Results Compared with the negative control group(siRNA-Control),siRNA-720,siRNA-1707 and siRNA-1690 had different inhibitory effects on the expression of B7-H3.B7-H3 inhibition of siRNA-1690 was significantly higher than that of siRNA-720 and siRNA-1707,and siRNA-1690 sequence was chosen for follow-up experiment.The results of CCK-8 cell viability assay showed that the proliferation ability of HUVECs decreased by 24%,22%(P>0.05,compared with 24 h)and 15%(P<0.05,compared with 48 h)respectively at 24 h,48 h and 72 h after B7-H3 knockout.The migration ability of B7-H3 for 24 h was significantly lower than that of siRNA-Control group(P<0.01).The results of three-dimensional cell culture showed that the angiogenic ability of HUVECs decreased significantly after si-B7-H3 knockdown of B7-H3 gene(P<0.01).Conclusion Targeting B7-H3 inhibits the growth,migration,and angiogenesis of human umbilical vein endothelial cells.

关 键 词：协同信号分子 血管生成 靶向药物 小干扰RNA 人脐静脉血管内皮细胞 细胞迁移 

分 类 号：R322.123[医药卫生—人体解剖和组织胚胎学] R329.28[医药卫生—基础医学] R364.3R392.11R394.2