NaCl胁迫对非洲菊幼苗生长与生理特性的影响  

EFFECT OF NACL STRESS ON THE GROWTH AND PHYSIOLOGICAL CHARACTERISTIC GERBERA JAMESONII SEEDLINGS

作　　者：宋俊志 许承轩 武文韬 张云 王靖涵 高青海 葛伟强 贾双双 SONG Junzhi;XU Chengxuan;WU Wentao;ZHANG Yun;WANG Jinghan;GAO Qinghai;GE Weiqiang;JIA Shuangshuang(College of Agriculture,Anhui Science and Technology University,Fengyang,Anhui 233100,China;Anhui Zhongyi Agricultural Technology Co.,Ltd.,Huaiyuan,Anhui 233000,China)

机构地区：[1]安徽科技学院农学院,安徽凤阳233100 [2]安徽中以农业科技有限公司,安徽怀远233000

出　　处：《井冈山大学学报（自然科学版）》2024年第3期43-50,共8页Journal of Jinggangshan University (Natural Science)

基　　金：安徽省教育厅重点项目(KJ2017A514);安徽省2018年度重点研究与开发计划项目(1804g07020176);安徽科技学院自然重点项目(2021zrzd10);安徽科技学院科研发展基金项目(811393);2021年安徽省大学生创新创业训练计划项目(S202110879220)。

摘　　要：为探究切花非洲菊(Gerbera jamesonii)‘柯蒂奶油’和‘安娜’幼苗的耐盐生理作用机制,本试验以非洲菊幼苗为试验材料,利用不同浓度(0、100、125、150、200 mmol/L)的Na Cl溶液模拟盐渍土,研究其对非洲菊幼苗生长及生理特性的影响。结果表明:当非洲菊‘柯蒂奶油’和‘安娜’的Na Cl浓度分别在≤100 mmol/L和≤125 mmol/L时,低盐胁迫刺激非洲菊幼苗叶片,通过提高超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)等保护酶的活性来清除植物体内积累的活性氧(ROS),保持了细胞质膜的稳定,维持正常的渗透平衡与细胞代谢,促进非洲菊地上、地下部鲜重和叶绿素含量的增加;当非洲菊‘柯蒂奶油’和‘安娜’的NaCl浓度分别在≥125 mmol/L和≥150 mmol/L时,高盐胁迫导致植物体内活性氧过量累积,丙二醛(MDA)含量迅速增加,渗透代谢平衡被打破,致使细胞失水,严重抑制非洲菊幼苗的生长。因此,非洲菊‘柯蒂奶油’幼苗可耐≤100 mmol/L的Na Cl胁迫,‘安娜’幼苗可耐≤125 mmol/L的Na Cl胁迫。In order to investigate the physiological mechanism of salt tolerance in the seedlings of cut flower Gerbera jamesonii'Curtis Cream'and'Anna',G.jamesonii seedlings as object,using NaCl solutions of different concentrations(0 mmol/L,100 mmol/L,125 mmol/L,150 mmol/L,200 mmol/L,100 mmol/L)to simulate the saline soil,its effects on the growth and physiological characteristics of G.jamesonii seedlings were studied.The results showed that low salt stress stimulated the leaves of G.jamesonii'Curtis Cream'and'Anna'seedlings at NaCl concentrations of≤100 and≤125 mmol/L,respectively,and promoted the accumulation of reactive oxygen species(ROS)in the plants by increasing the activities of protective enzymes such as superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT),and maintaining the stability of the plasma membrane.It maintained the stability of the plasma membrane,maintains normal osmotic balance and cellular metabolism,and promoted the increase of fresh weight and chlorophyll contents of G.jamesonii above and below ground.When the NaCl concentrations were≥125 and≥150 mmol/L,respectively,the high salt stress led to excessive accumulation of reactive oxygen species in the plants,and the contents of malondialdehyde(MDA)increased rapidly.The osmotic balance of metabolism was broken,resulting in cellular water loss and serious inhibition of the growth of G.jamesonii seedlings.Therefore,G.jamesonii'Curtis Cream'seedlings could tolerate≤100 mmol/L NaCl stress,and'Anna'seedlings could tolerate≤125 mmol/L NaCl stress.

关 键 词：非洲菊 单盐胁迫 生长发育 抗氧化酶 渗透调节物质 

分 类 号：Q945.78[生物学—植物学]