基于氮掺杂的碳点快速检测环境样品中2,4-二硝基苯酚  

Rapid detection of 2,4-dinitrophenol in environmental samples based on N-doped carbon dots

作　　者：柴艳 刘永丽 焦亚 马聪 刘桓嘉 葛国蓓 Chai Yan;Liu Yongli;Jiao Ya;Ma Cong;Liu Huanjia;Ge Guobei(Xinxiang Environmental Protection Science Design and Research Institute,Xinxiang 453000,China;College of Environment,Henan Normal University,Xinxiang 453007,China)

机构地区：[1]新乡市环境保护科学设计研究院,河南新乡453000 [2]河南师范大学环境学院,河南新乡453007

出　　处：《河南师范大学学报（自然科学版）》2024年第4期142-148,共7页Journal of Henan Normal University(Natural Science Edition)

基　　金：国家自然科学基金(22106039);河南师范大学国家级科研项目培育基金(2021PL23).

摘　　要：为快速灵敏检测2,4-二硝基苯酚(2,4-DNP),以丙二酸和聚丙烯酰胺为前驱体,通过绿色简便的一步水热法合成氮掺杂碳点(N-CDs).通过透射电子显微镜(TEM)、傅里叶变换红外光谱(FTIR)、紫外可见吸收光谱(UV-Vis)和荧光光谱对氮掺杂碳点的结构和光学性能进行了表征;并利用氮掺杂碳点构筑新型荧光探针检测环境样品中2,4-DNP,考察其检测效果和淬灭机理.结果表明,2,4-DNP可特异性识别并淬灭碳点的荧光,淬灭机理为内滤效应;探针的淬灭度与2,4-DNP质量浓度在0.050~10.000 mg/L范围内有良好的线性响应,检测限为0.015 mg/L;加标回收试验表明构建的方法能成功用于实际水样和土样中2,4-DNP的检测.N-doped fluorescent carbon dots(N-CDs)were prepared by using malonic acid and polyacrylamide as the precursors via a green simple one-step hydrothermal method to rapidly and sensitively detect 2,4-dinitrophenol(2,4-DNP).The structure and optical properties of N-CDs were characterized by transmission electron microscopy(TEM),fourier transform infrared spectroscopy(FTIR),ultraviolet-visible absorption spectroscopy(UV-Vis)and fluorescence spectroscopy.A novel fluorescent probe was constructed based on N-CDs to detect 2,4-DNP in environmental samples,and the sensitivity and quenching mechanism were further investigated.The results indicated that the fluorescence of N-CDs was specifically quenched by 2,4-DNP with the quenching mechanism of static quenching.There was a good linear relationship between the quenching effect of the probe and the 2,4-DNP concentration in the range of 0.050-10.000 mg/L,and the detection limit was 0.015 mg/L.The recovery experiments dedicated that the constructed method can be successfully utilized to analyze 2,4-DNP in actual water samples and soil samples.

关 键 词：碳点 荧光检测 2 4-二硝基苯酚 内滤效应 

分 类 号：O657[理学—分析化学]