黄缘短头叶蝉线粒体基因组及系统发育分析  

Analysis on complete mitochondrial genome and phylogeny of Iassus lateralis

作　　者：杨文欣 贺俊芳 田莉莉 王伟 李敏[3] 张斌[1] YANG Wenxin;HE Junfang;TIAN Lili;WANG Wei;LI Min;ZHANG Bin(College of Life Sciences and Technology,Inner Mongolia Normal University,Hohhot,Inner Mongolia 010022,China;Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu,Sichuan 610041,China;College of Biological Sciences and Technology,Taiyuan Normal University,Jinzhong,Shanxi 030619,China)

机构地区：[1]内蒙古师范大学生命科学与技术学院,内蒙古呼和浩特010022 [2]中国科学院成都生物研究所,四川成都610041 [3]太原师范学院生命科学与技术学院,山西晋中030619

出　　处：《福建农林大学学报（自然科学版）》2024年第4期433-442,共10页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金项目(32260122、31760632);内蒙古自然科学基金项目(2020MS03014);内蒙古自治区科技计划项目(2021GG0112);内蒙古师范大学基本科研业务费专项资金(2022JBXC016);内蒙古师范大学研究生科研创新基金项目(CXJJS22121)。

摘　　要：【目的】探究黄缘短头叶蝉(Iassus lateralis)的线粒体基因组结构及叶蝉亚科(Iassinae)的系统发育关系,为叶蝉的分类学、群体遗传学和进化学研究提供参考。【方法】通过高通量测序技术组装、拼接获得黄缘短头叶蝉线粒体基因组全序列,对其进行分析,并基于13个蛋白质编码基因(PCGs)和2个核糖体RNA基因(rRNAs)构建系统发育树。【结果】黄缘短头叶蝉线粒体基因组全长为15153 bp(GenBank登录号:OQ991898.2),由37个编码基因和1个控制区组成,37个编码基因包括13个PCGs、22个转运RNA基因(tRNAs)和2个rRNAs;碱基组成呈现明显的A+T偏向性;PCGs以ATN或TTG作为起始密码子,以TAA、TAG或T作为终止密码子;13个PCGs编码3624个氨基酸,其中,脯氨酸(Phe)的含量最多;22个tRNAs中,除trnS1缺失二氢尿嘧啶(DHU)臂外,其余均为典型的三叶草结构。系统发育分析表明,叶蝉亚科内的系统发育关系为:网脉叶蝉族(Krisinimi)单独聚为一支,短头叶蝉族(Iassini)、短板叶蝉族(Hyalojassini)和窄头叶蝉族(Batracomorphini)聚为一支。其中,黄缘短头叶蝉与褐盾短头叶蝉(I.dorsalis)聚为一支。【结论】叶蝉亚科内各族进化关系稳定,黄缘短头叶蝉的分类地位无较大变动,短板叶蝉族的分类地位需要用更多的样本数据进一步确定。【Objective】Exploring the mitochondrial genome structure of Iassus lateralis and phylogenetic relationship of Iassinae will provide reference for the taxonomy,population genetics,and evolutionary studies of Cicadellidae.【Method】The mitochondrial genome of I.lateralis was obtained by high-throughput sequencing technology,and its phylogenetic trees were constructed based on 13 protein-coding genes(PCGs)and 2 ribosomal RNA genes(rRNAs).【Result】The mitochondrial genome of I.lateralis(GenBank accession number OQ991898.2)was 15153 bp in length,with strong A+T preference,and contained 37 typical genes[13 PCGs,2 rRNAs and 22 transfer RNA genes(tRNAs)]and 1 control region.All PCGs were initiated with ATN or TTG,and ended with TAA,TAG or incomplete stop condon(T).The 13 PCGs encoded 3624 amino acids,with Phe being the most common one.Except for trnS1 lacking dihydrouracil(DHU)stems,the remaining 21 tRNAs could be folded into a typical cloverleaf secondary structure.Phylogenetic analysis showed that Krisinimi formed a separate clade,while Iassini,Hyalojassini and Batracomorphini gathered into another clade;within Iassini,I.lateralis and I.dorsalis formed a clade.【Conclusion】The evolutionary relationship of the Iassinae subfamily is stable,in which the taxonomic status of I.lateralis showed no significant variation,while more sample data are needed to further determine the taxonomic status of Hyalojassini.

关 键 词：黄缘短头叶蝉 叶蝉亚科 高通量测序 线粒体基因组 系统发育 

分 类 号：Q969.362[生物学—昆虫学]