李属植物线粒体基因组特征与系统发育分析  

Structural characterization and phylogenetic evolution analysis of mitochondrial genomes of Prunus plants

作　　者：陈欣雨 朱守玟 江转转 CHEN Xinyu;ZHU Shouwen;JIANG Zhuanzhuan(College of Life Sciences,Anqing Normal University/Provincial Key Laboratory of the Biodiversity Study and Ecology Conservation in Southwest Anhui,Anqing,Anhui 246133,China)

机构地区：[1]安庆师范大学生命科学学院/皖西南生物多样性研究与生态保护安徽省重点实验室,安徽安庆246133

出　　处：《福建农林大学学报（自然科学版）》2024年第4期491-500,共10页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：杂交水稻国家重点实验室开放课题(KF202204);安徽省大学生创新创业项目(S202310372084、S202310372079)。

摘　　要：【目的】李属植物种类庞杂,其线粒体基因组相关研究较少。利用比较基因组学对李属植物线粒体基因组进行分析,旨在为李属植物系统发育、种群鉴定等研究提供参考。【方法】基于NCBI数据库已发布的7种李属植物线粒体基因组序列,利用生物信息学的方法,对其基因组的特征、组成,基因转移,遗传变异,系统发育等方面进行分析。【结果】7种李属植物的线粒体基因组序列长度为422215(寒樱)~535727 bp(梅),GC含量均约为45%,编码序列数量相近。跨膜结构域分析显示,线粒体基因组部分开放阅读框可能包含信号肽。DNA从叶绿体迁移至线粒体分析显示,tRNA基因比rRNA基因更为保守。7种李属植物线粒体基因组密码子偏好性不强,但共线性较高,大多数蛋白质编码基因在进化过程中受纯化选择。线粒体全基因组和单基因系统发育分析显示,寒樱与浙闽樱桃、梅与山杏的亲缘关系更近,李与光核桃的亲缘关系也较为接近,此结果与植物学分类标准存在一致性。【结论】李属线粒体基因组是其全基因组的重要组成部分,其序列较为保守,可用于物种鉴定、系统发育等方面的研究。【Objective】Few researches were focused on the mitochondrial genome of Prunus despite its numerous and complex members.In this paper,comparative genomics was performed on the mitochondrial genome of Prunus to provide reference for their phylogeny and population identification.【Method】Based on the mitochondrial genome sequences of 7 Prunus species published in NCBI database,their characteristics,composition,gene transfer,genetic variation and phylogeny were analyzed by bioinformatics methods.【Result】The mitochondrial genome of the 7 Prunus species ranged from 422215(P.kanzakura)to 535727 bp(P.mume)in length,and they had an average GC content of about 45%and shared similar numbers of coding sequence.Transmembrane domain analysis showed that some open reading frames may contain signal peptides,and analysis on gene transfer from chloroplast to mitochondria suggested that the tRNA genes were more conserved than the rRNA genes.The mitochondrial genome codon of the 7 Prunus species showed weak codon bias,but had strong collinearity,and most of the protein-coding genes were under purification selection during evolution.Mitochondrial genome and single-gene phylogenetic analysis showed closer relationships between P.kanzakura and P.schneiderana,P.mume and P.sibirica,as well as P.salicina and P.mira,which were consistent with the botanical classification standard.【Conclusion】As an important part of plant whole genome,the mitochondrial genome of Prunus has relatively conserved sequence,and can be used for species identification,phylogeny and etc.

关 键 词：李属 线粒体基因组 重复序列 基因转移 系统发育 

分 类 号：S662.3[农业科学—果树学]