基于55K芯片小麦籽粒相关性状的QTL定位分析  

QTL Mapping Analysis of Grain Related Traits of Wheat Based on 55K Chip

作　　者：张津津 赵晓雪 刘萍 万家乐 陈心怡 陈璨 司红起[1,2] 李亮 马传喜[1,2] 卢杰 ZHANG Jinjin;ZHAO Xiaoxue;LIU Ping;WAN Jiale;CHEN Xinyi;CHEN Can;SI Hongqi;LI Liang;MA Chuanxi;LU Jie(Key Laboratory of Wheat Biology and Genetics Breeding in South Huanghuai Region,Ministry of Agriculture,Hefei 230036,China;School of Agronomy,Anhui Agricultural University,Hefei 230036,China;Wupu Farm of Suixi County,Suixi 235100,China)

机构地区：[1]农业部黄淮南部小麦生物学与遗传学育种重点实验室,安徽合肥230036 [2]安徽农业大学农学院,安徽合肥230036 [3]濉溪县五铺农场,安徽濉溪235100

出　　处：《华北农学报》2024年第3期8-14,共7页Acta Agriculturae Boreali-Sinica

基　　金：国家现代农业产业技术体系-国家小麦产业技术体系专项(CARS-03);安徽省重大科技专项(2021d06050003)。

摘　　要：为了进一步挖掘小麦籽粒相关性状的主效QTL位点,探索籽粒性状之间的遗传关系,利用籽粒性状差异较大的小麦品种安农859和武农988构建的124份DH群体为研究材料,分别测定2 a 7个环境下的粒长、粒宽及千粒质量表型值,开展籽粒性状多元回归分析,并基于DH群体的55K芯片数据进行籽粒相关性状QTL检测。结果表明,多元回归分析中,粒宽对千粒质量的贡献最大。通过完备区间作图对籽粒性状进行QTL定位,除6D和7B染色体外,其他19条染色体上共检测到69个有关籽粒性状的QTL,包括24个千粒质量QTL、28个粒长QTL、17个粒宽QTL,单个QTL的表型解释率为6.87%~27.74%。其中,7A染色体上粒长相关的Qgl.ahau-7A.1在7个环境及BLUP下均被检测到,表型解释率为9.48%~22.26%,加性效应为0.11~0.21 mm,物理区间4.91 Mb(AX-110430243~AX-110442528),可能为新的主效QTL。因此,Qgl.ahau-7A.1位点可作为后续精细定位和分子标记辅助育种重点关注的区域。In order to further explore the primary QTL loci for grain-related traits in wheat and explore the genetic relationships among grain traits,124 DH populations constructed from wheat varieties AN859 and WN988 with large differences in grain traits were utilized as research materials,The phenotypic values of grain length,grain width,and thousand grains weight were measured in seven environments over two years,respectively,to carry out the multiple regression analysis of grain traits,and QTL detection of grain-related traits was performed based on the 55K microarray data of the DH populations.The results showed that grain width contributed most to thousand grains weight in the multiple regression analysis.QTL localization for grain traits by complete interval mapping,a total of 69 QTLs related to grain traits were detected on 19 chromosomes except chromosome 6D and 7B,including 24 QTLs for thousand grains weight,28 QTLs for grain length,and 17 QTLs for grain width,with phenotypic interpretations of individual QTLs ranging from 6.87%to 27.74%.Among them,grain length-associated Qgl.ahau-7A.1 on chromosome 7A was detected under seven environments and BLUP,with a phenotypic interpretation rate of 9.48%-22.26%,an additive effect of 0.11-0.21 mm,and a physical interval of 4.91 Mb(AX-110430243-AX-110442528),for the new primary effector QTL.Therefore,the Qgl.ahau-7A.1 locus can be used as a region of focus for subsequent fine localization and molecular marker-assisted breeding.

关 键 词：小麦 籽粒性状 千粒质量 粒长 粒宽 QTL 

分 类 号：S512.1[农业科学—作物学]