1,3-氧硫戊环为受体的Hg^(2+)荧光探针的设计、合成及性能  

Design,Synthesis and Performance of Fluorescent Probe for Detection of Hg^(2+)With 1,3-Oxathiolane as Receptor

作　　者：梁庆祥 周五 吴爱斌[1,2,3] 舒文明 余维初 LIANG Qing-xiang;ZHOU Wu;WU Ai-bin;SHU Wen-ming;YU Wei-chu(Department of Chemistry,School of Chemistry and Environmental Engineering,Yangtze University,Jingzhou 434023,China;Unconventional Oil and Gas Collaborative Innovation Center,Yangtze University,Jingzhou 434023,China;Hubei Engineering Research Centers for Clean Production and Pollution Control of Oil and Gas Fields,Jingzhou 434023,China)

机构地区：[1]长江大学化学与环境工程学院化学系,湖北荆州434023 [2]长江大学非常规油气协同创新中心,湖北荆州434023 [3]油气田清洁生产与污染物控制湖北省工程研究中心,湖北荆州434023

出　　处：《光谱学与光谱分析》2024年第7期1913-1917,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(52274029)资助。

摘　　要：Hg^(2+)是毒性最强的重金属离子之一,会造成空气、土壤和水的污染,严重损害人体健康,开发有效的分析方法检测环境体系中的Hg^(2+)尤为重要。荧光探针因其灵敏度高、选择性好、响应速度快、可实时在线检测等优点,已广泛应用于Hg^(2+)检测。以Hg^(2+)促进硫代缩醛的去保护反应设计合成了一种全新的以1,3-氧硫戊环为受体的开启型Hg^(2+)荧光探针[2-(pyren-1-yl)-1,3-oxathiolane,POX],通过^(1)H NMR、^(13)C NMR和HRMS对POX结构进行表征,考察了POX在CH_(3)CH_(2)OH/H_(2)O中对Hg^(2+)的选择性、竞争性、浓度滴定、pH滴定、时间依赖性、检出限和识别机理等。研究结果表明,POX可在较宽的pH范围内对Hg^(2+)快速识别,并表现出高度的选择性和灵敏度;向POX中加入Hg^(2+)后,在386 nm处出现明显的荧光发射峰,表明POX对Hg^(2+)呈现出显著的荧光“开启”效应,其识别过程几乎不受其他金属离子干扰;荧光滴定实验表明POX在Hg^(2+)浓度为0~6.5μmol·L^(-1)范围内具有良好的线性响应(R^(2)=0.9994),检出限为0.168μmol·L^(-1),在实际水样中检测Hg^(2+)的RSD小于2.92%。由于POX合成简单、原料易得且pH适用范围较广,可作为定性和定量检测环境中Hg^(2+)的潜在工具。Hg^(2+)is one of the most toxic heavy metal ions,which can cause air,soil,and water pollution,seriously damaging human health.Therefore,developing effective analytical methods to detect Hg^(2+)in environmental systems is particularly important.Fluorescent probes have been widely used to detect Hg^(2+)due to their advantages,such as high sensitivity,good selectivity,fast response time,and real-time online detection.In this paper,a novel“turn-on”fluorescent probe(2-(pyren-1-yl)-1,3-oxathiolane,POX)with 1,3-oxathiolane as receptor was designed and synthesized based on Hg^(2+)-promoted deprotection reaction of thioacetal,and ^(1)H NMR,^(13)C NMR,and HRMS characterized its structure.The selectivity,competitiveness,concentration titration,pH titration,time dependence,the limit of detection,and recognition mechanism of POX for the detection of Hg^(2+)in CH_(3)CH_(2)OH/H_(2)O solution were investigated.The results showed that POX could quickly recognize Hg^(2+)in a wide pH range and exhibited high selectivity and sensitivity.Adding Hg^(2+)to the solution of POX resulted in a clear fluorescence emission peak at 386 nm,indicating that POX showed a remarkable“turn-on”fluorescence for Hg^(2+),and its recognition process was almost unaffected by other metal ions.Fluorescence titration experiments indicated that POX had a good linear response(R^(2)=0.9994)in the range of Hg^(2+)from 0~6.5μmol·L^(-1),with a detection limit of 0.168μmol·L^(-1).The RSD of POX for detecting Hg^(2+)in actual water samples was less than 2.92%.The simple synthesis,easy availability of raw materials,and wide pH applicability of POX suggested that itcould be used as a potential tool for the qualitative and quantitative detection of Hg^(2+)in the environment.

关 键 词：荧光探针 Hg^(2+) 1 3-氧硫戊环 荧光开启 

分 类 号：O657.3[理学—分析化学]