基于α7nAChR/NF-κB通路研究点按脾俞、胃俞穴对缓解慢性疲劳综合征大鼠外周炎症的效应机制  

Mechanism of effects of pressing Pishu(BL20)and Weishu(BL21)on alleviating peripheral inflammation in rats with chronic fatigue syndrome based on theα7nAChR/NF-κB pathway

作　　者：林巧婷 钟叶蓓 杨尚林 裴鑫 杨小慧 李铁浪[1] 李武[1] LIN Qiaoting;ZHONG Yebei;YANG Shanglin;PEI Xin;YANG Xiaohui;LI Tielang;LI Wu(School of Acupuncture-moxibustion,Tuina and Rehabilitation,Hunan University of Chinese Medicine,Changsha,Hunan 410208,China)

机构地区：[1]湖南中医药大学针灸推拿与康复学院,湖南长沙410208

出　　处：《湖南中医药大学学报》2024年第6期1027-1033,共7页Journal of Hunan University of Chinese Medicine

基　　金：湖南省自然科学基金项目(2023JJ30454);湖南省教育厅科学研究重点项目(22A0273);湖南中医药大学研究生创新课题项目(2022CX107)。

摘　　要：目的观察点按脾俞、胃俞穴对慢性疲劳综合征(chronic fatigue syndrome,CFS)模型大鼠疲劳状态、运动行为、外周炎症及α7烟碱型乙酰胆碱受体(α7 nicotinic acetylcholine receptor,α7nAChR)/核因子κB(nuclear factor-κB,NF-κB)信号表达的影响,探讨点按脾俞、胃俞穴对缓解CFS大鼠外周炎症的效应机制。方法先将32只SD大鼠随机分为空白组(8只)和造模组(24只)。造模组采用强迫负重游泳联合慢性应激刺激的方法建立CFS大鼠模型。造模成功后,将造模组大鼠随机分为模型组、点按组、α7nAChR激动剂组,每组8只。空白组、模型组予腹腔注射等体积生理盐水;点按组以自制按法刺激仪点按双侧脾俞、胃俞穴,20 min/次,并予腹腔注射等体积生理盐水;α7nAChR激动剂组予腹腔注射α7nAChR激动剂PNU-282987(每次2.4 mg/kg)。每组每日干预1次,连续14 d。记录各组大鼠一般情况半定量评分、力竭游泳时间和旷场实验运动距离;计算大鼠脾脏指数和胸腺指数;采用ELISA法检测大鼠血清中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)、白细胞介素-6(interleukin-6,IL-6)的含量;Western blot检测大鼠脾脏组织α7nAChR、NF-κB p65、NF-κB p-p65、TNF-α的蛋白表达水平;采用qPCR法检测α7nAChR、NF-κB p65、TNF-α的mRNA表达水平。结果与空白组比较,模型组一般情况半定量评分,血清TNF-α、IL-1β、IL-6含量,脾脏组织中NF-κB p-p65、NF-κB p65、TNF-α蛋白表达水平及NF-κB p65、TNF-αmRNA表达量均升高(P<0.01);力竭游泳时间、旷场实验运动距离、脾脏指数、胸腺指数、脾脏组织中α7nAChR蛋白表达水平及mRNA表达量均降低(P<0.05,P<0.01)。与模型组比较,点按组与α7nAChR激动剂组一般情况半定量评分,血清TNF-α、IL-1β、IL-6含量,脾脏组织中NF-κB p-p65、NF-κB p65、TNF-α蛋白表达水平及NF-κB p65、TNF-αmRNA表达量均降低(P<0.05,P<0.01);力竭游泳Objective To observe the effects of pressing Pishu(BL20)and Weishu(BL21)on the fatigue state,locomotor behaviors,peripheral inflammation,andα7 nicotinic acetylcholine receptor(α7nAChR)/nuclear factor-κB(NF-κB)signaling expression of chronic fatigue syndrome(CFS)model rats,and to explore the mechanism of effect of this therapy on the alleviation of peripheral inflammation in CFS rats.Methods A total of 32 SD rats were randomized into blank group(n=8)and modeling group(n=24).In the modeling group,a CFS model was established by forced weight-bearing swimming combined with chronic stress stimulation.After successful modeling,the rats in the modeling group were randomly subdivided into model,acupoint-pressing,andα7nAChR agonist groups,with eight rats in each group.The blank and model groups were injected intraperitoneally with an equal volume of saline;the acupoint-pressing group was subjected to the bilateral press on Pishu(BL20)and Weishu(BL21)using a homemade pressing stimulator for 20 min each time as well as intraperitoneal injection of an equal volume of saline;theα7nAChR agonist group was injected intraperitoneally with theα7nAChR agonist PNU-282987(2.4 mg/kg per time).Each group was intervened once daily for continuous 14 days.The semi-quantitative score of the general condition,swimming time to exhaustion,and exercise distance in the open-field test were recorded in each group;the spleen index and thymus index were calculated;the content of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),and interleukin-6(IL-6)in rat serum were checked using ELISA;the protein expression levels ofα7nAChR,NF-κB p65,NF-κB p-p65,and TNF-αin the rat spleen tissue were examined by Western blot;the mRNA expression levels ofα7nAChR,NF-κB p65,and TNF-αwere determined by qPCR.Results Compared with the blank group,the semi-quantitative score of the general condition,serum content of TNF-α,IL-1β,and IL-6,as well as the protein expression levels of NF-κB p-p65,NF-κB p65,and TNF-αand the mRNA expression levels o

关 键 词：慢性疲劳综合征 点按法 脾俞 胃俞 α7烟碱型乙酰胆碱受体/核转录因子κB通路 外周炎症 

分 类 号：R245[医药卫生—针灸推拿学]