儿童重症肺炎与IL-6-174G/C IL-6-572C/G及IL-6-597G/A位点基因多态性相关性研究  

Study on the correlation between severe pneumonia in children and gene polymorphisms of interleukin-6-174G/C,-572C/G,-597G/A

作　　者：梁建华 罗红芳 杨祝青 陈通业 潘有松 吴丽萍 LIANG Jian-hua;LUO Hong-fang;YANG Zhu-qing;CHEN Tong-ye;PAN You-song;WU Li-ping(Department of Pediatrics,Second Affiliated Hospital,Guizhou Medical University,Kaili,Guizhou 556000,China)

机构地区：[1]贵州医科大学第二附属医院儿科,贵州凯里556000

出　　处：《中国妇幼保健》2024年第12期2263-2266,共4页Maternal and Child Health Care of China

基　　金：贵州省卫生健康委科学技术基金项目(gzwjkj2023-422)。

摘　　要：目的 探讨IL-6-174G/C、IL-6-597G/A及IL-6-572C/G位点基因的多样性与未成年人患肺炎(重度)的关系。方法 选取2020年1月—2022年10月贵州医科大学第二附属医院收治的100例重症肺炎患儿作为研究组;另选取同期于该院进行体检的100例轻度肺炎患儿作为对照组,按照性别、年龄等与研究组匹配。比较重症肺炎与IL-6-174G/C、IL-6-572C/G及IL-6-597G/A位点基因多态性的关系。结果 研究组白细胞计数(WBC)水平为(8.63±2.76)×10^(9)/L,IL-6表达水平为(58.67±8.91)ng/L;对照组WBC水平为(6.23±1.31)×10^(9)/L,IL-6表达水平为(38.23±5.16)ng/L,两组比较差异均有统计意义(均P<0.05)。IL-6-174G/C位点:两组中可见G/G和C/G 2种基因型,研究组G/G、C/G型频率分别为98.43%、1.57%,对照组G/G、C/G型频率分别为97.25%、2.75%,未检测到C/C型,G→C等位基因突变频率极低。IL-6-597 G/A位点:结果中未见-597位点G-A基因的突变。IL-6-572C/G位点:两组均可见3种基因型。经检验,与Hardy-Weinberg平衡结论相符合,提示结果有群体的代表性(研究组χ^(2)=7.156,对照组χ^(2)=12.509,均P<0.05)。两组患儿等位基因的频率分布、基因型频率的分布比较,差异有统计学意义(P<0.05)。研究组基因型C/C的患儿C-反应蛋白(CRP)表达水平为(57.73±12.71)mg/L,血清IL-6表达水平为(327.82±122.72)ng/L;基因型G/G+C/G的患儿CRP表达水平为(59.89±13.52)mg/L,血清IL-6表达水平为(342.64±123.54)ng/L,差异有统计学意义(P<0.05)。研究组基因型C/C的患儿D-二聚体表达水平(0.73±0.19)mg/L、PCT(0.48±0.11)ng/ml、LDH(1 047.53±322.81)U/L,基因型G/G+C/G的患儿D-二聚体(0.83±0.25)mg/L、PCT(0.53±0.18)ng/ml、LDH(1 051.33±328.69)U/L,差异无统计学意义(P<0.05)。而对照组基因型C/C的患儿CRP(4.62±1.51)mg/L、血清IL-6水平(112.35±42.35)ng/L和基因型G/G+C/G的患儿CRP(4.89±1.72)mg/L、血清IL-6水平(118.57±46.57)ng/L,基因型C/C的患儿D-二聚体(0.23±0.16)mg/L、PCT(0.24±0.15)Objective To investigate the correlation between severe pneumonia in children and IL-6-174G/C,IL-6-572C/C,IL-6-597G/A locus gene polymorphisms.Methods Study group(severe pneumonia group)subjeels:one hundred children with severe pneu-monia admitted to our hospital from January 2020 to October 2022;Control subjects:one hundred children with mild pneumonia who under-went physical examination in our hospital during the same period,matched with the study group according to gender,age,etc.The relation-ship between IL-6-174G/C,IL-6-572C/C,IL-6-597G/A gene polymorphisms and severe pneumonia in children was compared between the two groups.Results There was no significant dfference in general conditions between the two groups(P>0.05).The WBC count(8.63±2.76)and IL-6(58.67±8.91)in the study group were signifcandly higher than those in the control group(6.23±1.31)and IL-6(38.23±5.16),there were stistically significant diferences(P<0.05);IL-6-174C/C site:G/in the 2 groups Two genolypes,G and C/G.The frequencies of G/G and C/G types in the severe pneumonia group were 98.43%and 1.57%,respectively.The frequencies of G/G and C/G lypes in the control group were 97.25%and 2.75%,respectively.No C/C type was detected,C→C The frequency of alelie mutations is extremely low.IL-6-597 G/A site:There was no G-A gene mutation at-597 site in the resuls.IL-6-572C/G locus:3 geno-types can be seen in both groups,After testing,it meets the Hardy-W einberg balance and is representative of the group(χ^(2)=7.156 in the severe pneumonia group,χ^(2)=12.509 in the control group,all P>0.05).There was a statistically significant difference in the distribution of genolype frequency and alele frequency between the tro groups(P<0.05).In the study group,CRP(57.73±12.71)and IL-6(327.82±122.72)in children with genotype C/C and CRP(59.89±13.52)and IL-6(342.64±123.54)in children with genotype C/G+C/C,the data were significantly different.Stutistically significant(P<0.05);In the study group,D-dimerization value(0.73±0.19),PCT(0.48±0.11),LDH(1047.53

关 键 词：基因多态性 儿童重症肺炎 IL-6-174G/C IL-6-572C/G IL-6-597G/A 

分 类 号：R446.1[医药卫生—诊断学]