miRNA-5582在卵巢癌组织中的表达及对卵巢癌细胞增殖和侵袭的影响  

Expression of miRNA-5582 in ovarian cancer tissue and its effect on the proliferation and invasion of ovarian cancer cells

作　　者：邵玉婷[1] 彭浩[1] 万淑琼[1] 曹欢 郑敏 潘春燕[1] SHAO Yuting;PENG Hao;WAN Shuqiong;CAO Huan;ZHENG Min;PAN Chunyan(Department of Obstetrics and Gynecology,Huangshi Central Hospital(Affiliated Hospital ofHubei Institute of Technology),Huangshi 435000,Hubei,China;Department of Gynecology,Cancer Hospital Affiliated to Sun Yat-sen University,Guangzhou 510062,Guangdong,China)

机构地区：[1]黄石市中心医院(湖北理工学院附属医院)妇产科,湖北黄石435000 [2]中山大学附属肿瘤医院妇科,广州510062

出　　处：《中国性科学》2024年第6期60-65,共6页Chinese Journal of Human Sexuality

基　　金：国家自然科学基金项目(81872434)。

摘　　要：目的观察微小RNA(miRNA)-5582对卵巢癌SK-OV-3细胞增殖和侵袭的影响并探讨其可能的机制。方法采用加利福尼亚大学圣克鲁兹分校(UCSC)数据库分析miRNA-5582在卵巢癌组织中的表达及其与卵巢癌患者临床分期的关系。采用实时荧光定量聚合酶链反应(qRT-PCR)检测卵巢癌细胞株A2780、HO-8910、OC3、SK-OV-3和正常卵巢上皮细胞株IOSE80中miRNA-5582的表达,miRNA-5582表达最低的SK-OV-3细胞株分为对照组和miRNA-5582组,分别转染miR-NC模拟物和miRNA-5582模拟物。MTS法和Transwell实验分别检测SK-OV-3细胞增殖及侵袭。以microRNA.org和miRWalk3.0数据库检索miRNA-5582的靶基因,双荧光素酶报告基因实验验证miRNA-5582的靶基因。qRT-PCR和Western blot法检测靶基因表达。结果miRNA-5582在卵巢癌组织中的表达显著低于正常组织(P<0.01)。miRNA-5582表达水平与卵巢癌患者的临床分期呈负相关(P<0.01)。miRNA-5582在卵巢癌细胞株中表达均显著低于IOSE80细胞(P<0.05),SK-OV-3细胞中miRNA-5582表达最低(P<0.01)。对照组和miRNA-5582组的miRNA-5582相对表达量为1.42±0.73和10.46±0.91,转染体系构建成功(P<0.01)。miRNA-5582组SK-OV-3细胞活性在第3、第4和第5天显著低于对照组(P<0.05)。对照组和miRNA-5582组侵袭细胞数分别为(49.48±3.78)和(15.77±3.40)个,miRNA-5582组细胞侵袭数量显著少于对照组(P<0.01)。miRNA-5582的靶基因是丝氨酸/精氨酸富集剪接因子3(SRSF3)(P<0.01)。miRNA-5582组SK-OV-3细胞SRSF3基因表达显著低于对照组(P<0.01)。结论miRNA-5582在卵巢癌组织和细胞株中低表达,上调miRNA-5582通过抑制SRSF3基因表达降低卵巢癌SK-OV-3细胞的增殖和侵袭能力,miRNA-5582可能为卵巢癌的防治提供新的靶点。Objective To observe the effect of microRNA(miRNA)-5582 on the proliferation and invasion of ovarian cancer SK-OV-3 cells and explore its possible mechanism.Methods The University of California,Santa Cruz(UCSC)database was used to analyze miRNA-5582 expression in ovarian cancer tissue and its relationship with the clinical stage of ovarian cancer patients.Quantitative reverse transcription polymerase chain reaction(qRT-PCR)was used to analyze miRNA-5582 expression in ovarian cancer cell lines A2780,HO-8910,OC3,SK-OV-3 and normal ovarian epithelial cell lines IOSE80.The SK-OV-3 cell line with the lowest expression of miRNA-5582 were divided into control group and miRNA-5582 group,and were transfected with miR-NC mimic and miRNA-5582 mimic,respectively.MTS method and transwell assay were used to detect the proliferation and invasion of SK-OV-3 cells,respectively.The target gene of miRNA-5582 was retrieved from microRNA.org and miRWalk 3.0 databases.Dual luciferase reporter assay was used to verify the target gene of miRNA-5582.qRT-PCR and western blot were used to detect target gene expression.Results The expression of miRNA-5582 in ovarian cancer tissues was significantly lower than that in normal tissues(P<0.01).The expression level of miRNA-5582 was negatively correlated with the clinical stage of ovarian cancer patients(P<0.01).The expression of miRNA-5582 in ovarian cancer cell lines was all lower than that of IOSE80 cells(P<0.05),and the expression of miRNA-5582 in SK-OV-3 cells was the lowest(P<0.01).The relative expression of miRNA-5582 in the control group and miRNA-5582 group was 1.53±0.82 and 9.52±1.08,and the transfection system was successfully constructed(P<0.01).The cell activity of SK-OV-3 cells in the miRNA-5582 group was significantly lower than that of the control group on the third,fourth,and fifth day(P<0.05).The number of invasive cells in the control group and miRNA-5582 group were 49.48±3.78 and 15.77±3.40,respectively,and the number of invasive cells in the miRNA-5582 group was signific

关 键 词：miRNA-5582 卵巢癌 丝氨酸/精氨酸富集剪接因子3 细胞增殖 细胞侵袭 

分 类 号：R711[医药卫生—妇产科学]