腕踝针对七氟烷诱发老龄大鼠认知功能障碍及海马p-PLC_(γ)表达的影响  

Influence of ankle acupuncture on cognitive impairment caused by sevoflurane in elderly rats and expression of hippocampal phosphorylated phospholipase C_(γ)

作　　者：康伟亚 顼兴改 何涛 程志鸿 赵亮 Kang Weiya;Xu Xinggai;He Tao;Cheng Zhihong;Zhao Liang(Department of Anesthesia and Perioperative Medicine,Hebei Provincial Hospital of Traditional Chinese Medicine,Shijiazhuang 050000,China;Department of Neurology,Gaocheng Hospital of Integrated Traditional Chinese and Western Medicine,Shijiazhuang 052160,China;Department of Cardiothoracic Surgery,Hebei Hospital of Traditional Chinese Medicine,Shijiazhuang 050000,China)

机构地区：[1]河北省中医院麻醉与围术期医学科,石家庄050000 [2]河北省石家庄市藁城中西医结合医院神经内科,石家庄052160 [3]河北省中医院心胸外科,石家庄050000

出　　处：《中华麻醉学杂志》2024年第5期564-567,共4页Chinese Journal of Anesthesiology

基　　金：河北省中医药管理局科研计划项目(2022347)。

摘　　要：目的评价腕踝针对七氟烷诱发老龄大鼠认知功能障碍及海马磷酸化磷脂酶C_(γ)(p-PLC_(γ))表达的影响。方法SPF级健康老龄雄性SD大鼠15只,18月龄,体质量530~600 g,采用随机数字表法分为3组(n=5):假手术组(S组)、认知功能障碍组(CD组)和腕踝针组(A组)。采用吸入3%七氟烷与空气混合气体的方法制备大鼠认知功能障碍模型,A组麻醉前取右侧后肢下1-3区行腕踝针,针刺时间30 min,S组行假刺激。2 d后采用Morris水迷宫实验评价大鼠认知功能,随后麻醉大鼠处死取海马组织,HE染色和尼氏染色法观察病理学结果,采用Western blot法检测p-PLC_(γ)、磷酸化钙调蛋白依赖性蛋白激酶Ⅱ(p-CaMKⅡ)和磷酸化肌醇-1,4,5-三磷酸受体(p-IP3R)的表达。结果与S组比较,CD组和A组逃避潜伏期延长,穿越原平台位置次数减少,海马p-PLC_(γ)、p-CaMKⅡ和p-IP3R表达上调(P<0.05);与CD组比较,A组逃避潜伏期缩短,穿越原平台位置次数增加,海马p-PLC_(γ)、p-CaMKⅡ和p-IP3R表达下调(P<0.05)。A组大鼠海马病理学损伤程度较CD组减轻。结论腕踝针可减轻七氟烷诱发老龄大鼠认知功能障碍,机制与其抑制海马p-PLC_(γ)表达有关。Objective To evaluate the influence of ankle acupuncture on the cognitive impairment caused by sevoflurane in elderly rats and the expression of hippocampal phosphorylated phospholipase C_(γ)(p-PLC_(γ)).Methods Fifteen SPF-grade healthy male Sprague-Dawley rats,aged 18 months,weighing 530-600 g,were allocated into 3 groups(n=5 each)using a random number table method:sham surgery group(S group),cognitive dysfunction group(CD group)and ankle acupuncture group(A group).The cognitive dysfunction model was prepared by inhaling the mixture of 3% sevoflurane and air,with ankle acupuncture applied to the right hind limb 1-3 region for 30 min prior to anesthesia in A group.S group received sham stimulation.Two days later,the cognitive function was assessed using the Morris water maze test.The rats were subsequently sacrificed and hippocampal tissues were collected for microscopic examination of the pathological changes(using HE staining and Nissl staining)and for determination of the expression of p-PLC_(γ),phosphorylated calcium/calmodulin-dependent protein kinase Ⅱ(p-CaMKⅡ)and phosphorylated inositol-1,4,5-trisphosphate receptor(p-IP3R).Results Compared with S group,the escape latency was significantly prolonged,the number of crossing the original platform quadrant was reduced,and the expression of p-PLC_(γ),p-CaMKII and p-IP3R was up-regulated in CD group and A group(P<0.05).Compared with CD group,the escape latency was significantly shortened,the number of crossing the original platform quadrant was increased,and the expression of p-PLC_(γ),p-CaMKII and p-IP3R was down-regulated in A group(P<0.05).The pathological changes were significantly reduced in A group compared with CD group.Conclusions Ankle acupuncture can ameliorate cognitive dysfunction induced by sevoflurane in elderly rats,and the mechanism is related to inhibition of hippocampal p-PLC_(γ)expression.

关 键 词：腕踝针疗法 麻醉药 吸入 老年人 认知功能障碍 海马 磷脂酶C_(γ) 

分 类 号：R614[医药卫生—麻醉学]