Recent progress in online detection methods of bioaerosols  

作　　者：Taicheng An Zhishu Liang Zhen Chen Guiying Li 

机构地区：[1]Guangdong-Hong Kong-Macao Joint Laboratory for Contaminants Exposure and Health,Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control,Institute of Environmental Health and Pollution control,Guangdong University of Technology,Guangzhou 510006,China [2]Guangzhou Key Laboratory of Environmental Catalysis and Pollution Control,Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control,School of Environmental Science and Engineering,Institute of Environmental Health and Pollution Control,Guangdong University of Technology,Guangzhou 510006,China

出　　处：《Fundamental Research》2024年第3期442-454,共13页自然科学基础研究（英文版）

基　　金：financially supported by National Natural Science Foundation of China(U1901210,42177410 and 42130611);Science and Technology Project of Guangdong Province,China(2021A0505030070);Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program(2017BT01Z032);Science and Technology Program of Guangzhou(202201010684);and Young S&T Talent Training Program of Guangdong Provincial Association for S&T(GDSTA),China(2022QNRC23).

摘　　要：The aerosol transmission of coronavirus disease in 2019,along with the spread of other respiratory diseases,caused significant loss of life and property;it impressed upon us the importance of real-time bioaerosol detection.The complexity,diversity,and large spatiotemporal variability of bioaerosols and their external/internal mixing with abiotic components pose challenges for effective online bioaerosol monitoring.Traditional methods focus on directly capturing bioaerosols before subsequent time-consuming laboratory analysis such as culture-based methods,preventing the high-resolution time-based characteristics necessary for an online approach.Through a comprehensive literature assessment,this review highlights and discusses the most commonly used real-time bioaerosol monitoring techniques and the associated commercially available monitors.Methods applied in online bioaerosol monitoring,including adenosine triphosphate bioluminescence,laser/light-induced fluorescence spectroscopy,Raman spectroscopy,and bioaerosol mass spectrometry are summarized.The working principles,characteristics,sensitivities,and efficiencies of these real-time detection methods are compared to understand their responses to known particle types and to contrast their differences.Approaches developed to analyze the substantial data sets obtained by these instruments and to overcome the limitations of current real-time bioaerosol monitoring technologies are also introduced.Finally,an outlook is proposed for future instrumentation indicating a need for highly revolutionized bioaerosol detection technologies.

关 键 词：Airborne microorganisms Real-time monitoring Adenosine triphosphate luminescence Laser/light-induced fluorescence Raman spectroscopy Bioaerosol mass spectrometry 

分 类 号：X51[环境科学与工程—环境工程]