紫苏叶提取物对糖尿病肾脏病大鼠肾纤维化及Notch/Snail1信号通路的影响  

Effects of Perilla leaf extract on renal fibrosis and Notch/Snail1 signaling pathway in diabetic kidney disease rats

作　　者：张清[1] 叶晓萍[2] 胥勋梅[1] 张栋珉 钱娴静 Zhang Qing;Ye Xiaoping;Xu Xunmei;Zhang Dongmin;Qian Xianjing(Department of Endocrinology,The Third Hospital of Mianyang(Sichuan Mental Health Center),Mianyang 621000,China;Department of Outpatient,Mianyang Hospital of TCM,Mianyang 621000,China)

机构地区：[1]绵阳市第三人民医院(四川省精神卫生中心)内分泌科,绵阳621000 [2]绵阳市中医医院门诊部,绵阳621000

出　　处：《国际内分泌代谢杂志》2024年第3期150-154,211,共6页International Journal of Endocrinology and Metabolism

基　　金：绵阳市卫生健康委医学科研课题(202040)。

摘　　要：目的分析紫苏叶提取物对糖尿病肾脏病大鼠肾纤维化及Notch/锌指蛋白转录因子1(Snail1)信号通路的影响。方法选取40只大鼠,10只作为空白组,其余30只建立糖尿病肾脏病大鼠模型,共有29只大鼠建模成功,将其分为模型组9只、二甲双胍组10只、紫苏叶提取物组10只。二甲双胍组给予二甲双胍,紫苏叶提取物组给予紫苏叶提取物,空白组、模型组不做处理,干预14 d后观察大鼠变化。结果与空白组相比,模型组、二甲双胍组、紫苏叶提取物组血糖、尿素氮(BUN)、肌酐(Scr)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、Ⅳ型胶原(Ⅳ-C)、Ⅲ型前胶原(PCⅢ)、透明质酸(HA)、层黏连蛋白(LN)水平、Notch、Snail1 mRNA表达量及相对表达量上升(P均<0.05);与模型组相比,二甲双胍组、紫苏叶提取物组血糖、BUN、SCr、ALT、AST、Ⅳ-C、PCⅢ、HA、LN水平、Notch、Snail1 mRNA表达量及相对表达量下降(P均<0.05);二甲双胍组、紫苏叶提取物组血糖、BUN、SCr、ALT、AST、Ⅳ-C、PCⅢ、HA、LN水平、Notch、Snail1 mRNA表达量及相对表达量水平对比差异较小(P均>0.05)。结论糖尿病肾脏病大鼠经紫苏叶提取物干预后肾纤维化减轻,Notch/Snail1信号通路受抑。Objective To analyze the effects of Perilla leaf extract on renal fibrosis and Notch/zinc finger protein transcription factor 1(Snail1)signaling pathway in diabetic kidney disease rats.Methods 40 rats were selected,10 were used as blank group,and the remaining 30 rats were established with diabetic kidney disease model.A total of 29 rats were successfully modeled,and they were divided into model group 9,metformin group 10,and Perilla leaf extract group 10.The metformin group was given,the Perilla leaf extract group was given,the blank group and the model group were not treated,and the changes were observed after 14 days of intervention.Results Compared to the blank group,Levels of blood glucose,urea nitrogen(BUN),creatinine(Scr),alanine aminotransferase(ALT),aspartate aminotransferase(AST),type IV collagen(Ⅳ-C),typeⅢprocollagen(PCⅢ),hyaluronic acid(HA),laminin(LN),Notch and Snail1 in model group,metformin group and Perilla leaf extract group mRNA expression level and relative expression level were increased(P<0.05).Compared with model group,the levels of blood glucose,BUN,SCr,ALT,AST,Ⅳ-C,PCⅢ,HA,LN,Notch and Snail1 mRNA expression and relative expression of metformin group and Perilla leaf extract group were decreased(P<0.05).The mRNA expression levels of blood glucose,BUN,SCr,ALT,AST,Ⅳ-C,PCⅢ,HA,LN,Notch and Snail1 and their relative expression levels were significantly different between the metformin group and Perilla leaf extract group(P>0.05).Conclusion After treatment with Perilla leaf extract,renal fibrosis was alleviated and Notch/Snail1 signaling pathway was inhibited.

关 键 词：紫苏叶提取物 链脲佐菌素 糖尿病肾脏病 肾纤维化 Notch/锌指蛋白转录因子1 

分 类 号：R285.5[医药卫生—中药学]