基于高通量测序的三峡库区重庆段黄胸鼠肠道菌群分析  

Gut bacteria of Rattus tanezumi in Chongqing section of Three Gorges Reservoir area,China based on high throughput sequencing

作　　者：王玲[1,2] 肖汉森 何亚明 李志峰[1,2] 赵婷[1,2] 段刚 WANG Ling;XIAO Hansen;HE Yaming;LI Zhifeng;ZHAO Ting;DUAN Gang(Chongqing Center for Disease Control and Prevention,Chongqing 400707,China;不详)

机构地区：[1]重庆市疾病预防控制中心,重庆400707 [2]高致病性病原微生物重庆市重点实验室,重庆400707

出　　处：《中国微生态学杂志》2024年第5期504-511,517,共9页Chinese Journal of Microecology

基　　金：重庆市自然科学基金面上项目(CSTB2022NSCQ-MSX0572);重庆市英才计划(cstc2022ycjh-bgzxm0251)。

摘　　要：目的研究三峡库区重庆段黄胸鼠肠道细菌的群落结构和多样性,初步了解库区黄胸鼠携带病原体的本底资料。方法对三峡库区重庆段32例黄胸鼠的粪便样品进行16SrDNA高通量测序,分析黄胸鼠肠道菌群多样性、丰度和群落结构。结果三峡库区重庆段黄胸鼠肠道菌群主要以厚壁菌门、拟杆菌门和变形菌门为主。在属水平上,优势菌属包括乳杆菌属、Cellulosilyticum、八叠球菌属等。不同县(区)黄胸鼠肠道菌群结构不同,丰都县(FD)和陵区(FL)的菌群结构相似度较高,巫山县(WS)、开州区(KZ)、忠县(ZX)、奉节县(FJ)和云阳县(YY)5个区县群落结构相似度较高;三峡库区重庆段黄胸鼠肠道菌群结构相似度秋季明显高于春季。32份鼠粪样本测序结果提示含有葡萄球菌属、双歧杆菌属、沙门氏菌属、螺杆菌属等致病菌和条件致病菌。结论初步了解了三峡库区重庆段黄胸鼠的肠道菌群特征以及库区内地域性、季节性差异,黄胸鼠肠道中携带大量的致病菌和条件致病菌,应引起高度重视。Objective To reveal the structure and diversity of microbial community in guts of Rattus tanezumi in Chongqing section of the Three Gorges Reservoir(TGR)area,and grasp the background data of pathogens carried by Rattus tanezumi in the reservoir area.Methods High throughput sequencing of 16S rRNA V3-V4 regions was performed on 32 fecal samples of Rattus tanezumi from the Chongqing section of the TGR area,followed by species annotation and analysis of gut bacterial diversity,abundance and community structure.Results The gut bacterial communities of Rattus tanezumi in the Chongqing section of the TGR area mainly included three phyla,namely Firmicutes,Bacteroidetes and Proteobacteria.At the genus level,the dominant bacterial genera were Lactobacillus,Lachnospiraceae_NK4A136_group and Cellulosilyticum.The similarity of gut bacterial communities in Rattus tanezumi varied among different counties(districts).The similarity of the rodent gut bacterial communities between Fengdu County(FD)and Fuling County(FL)were higher,while Yunyang(YY),Zhongxian(ZX),Kaizhou(KZ),Fengjie(FJ),and Wushan(WS)counties showed higher similarity in the gut bacterial community structure of the rodents.The similarity of bacterial community structure in autumn was higher than in spring.The sequencing results of 32 rodent fecal samples indicated the presence of pathogenic and opportunistic bacteria such as Staphylococcus,Bifidobacterium,Salmonella and Helicobacter.Conclusion The results provided a preliminary understanding to the gut microbiota characteristics of Rattus tanezumi as well as the regional and seasonal differences in the Chongqing section of the TGR area;Rattus tanezumi carry a large number of pathogenic and opportunistic bacteria in their intestines,which should be highly valued.

关 键 词：三峡库区 黄胸鼠 高通量测序 肠道菌群 物种多样性 

分 类 号：R378[医药卫生—病原生物学] S443[医药卫生—基础医学]