In‑depth proteome characterization of endometrium and extraembryonic membranes during implantation in pig  

作　　者：Maria A.Gil Josep M.Cambra Heriberto Rodriguez‑Martinez Cristina Cuello Inmaculada Parrilla Emilio A.Martinez 

机构地区：[1]Department of Medicine and Animal Surgery,International Excellence Campus for Higher Education and Research“Campus Mare Nostrum”,University of Murcia,Murcia,Spain [2]Institute for Biomedical Research of Murcia(IMIB-Pascual Parrilla),Murcia,Spain [3]Technical University of Munich,Munich,Germany [4]Department of Biomedical&Clinical Sciences(BKV),BKH/Obstetrics&Gynaecology,Linkoping University,Linkoping,Sweden

出　　处：《Journal of Animal Science and Biotechnology》2024年第3期1027-1055,共29页畜牧与生物技术杂志（英文版）

基　　金：This research was funded by the MCIN/AEI/https://doi.org/10.13039/501100011033,ERDF(PID2022137645OB-I00),Madrid,Spain;Fundacion Seneca(19892/GERM/15),Murcia,Spain;the Swedish Research Council FORMAS(Project 2019-00288),Stockholm,Sweden.

摘　　要：Background Proteome characterization of the porcine endometrium and extraembryonic membranes is important to understand mother-embryo cross-communication.In this study,the proteome of the endometrium and cho-rioallantoic membrane was characterized in pregnant sows(PS)during early gestation(d 18 and 24 of gestation)and in the endometrium of non-pregnant sows(NPS)during the same days using LC-MS/MS analysis.The UniProtKB database and ClueGO were used to obtain functional Gene Ontology annotations and biological and functional networks,respectively.Results Our analysis yielded 3,254 and 3,457 proteins identified in the endometrium of PS and NPS,respectively;of these,1,753 being common while 1,501 and 1,704 were exclusive to PS and NPS,respectively.In addition,we iden-tified 3,968 proteins in the extraembryonic membranes of PS.Further analyses of function revealed some proteins had relevance for the immune system process and biological adhesion in endometrium while the embryonic chorion displayed abundance of proteins related to cell adhesion and cytoskeletal organization,suggesting they dominated the moment of endometrial remodeling,implantation and adhesion of the lining epithelia.Data are available via Pro-teomeXchange with identifier PXD042565.Conclusion This is the first in-depth proteomic characterization of the endometrium and extraembryonic mem-branes during weeks 3 to 4 of gestation;data that contribute to the molecular understanding of the dynamic environ-ment during this critical period,associated with the majority of pregnancy losses.

关 键 词：ENDOMETRIUM Extraembryonic membranes IMPLANTATION PIG PROTEOME 

分 类 号：S828[农业科学—畜牧学]