白银市5岁以下儿童流感病毒核酸阴性的流感样病例呼吸道病原谱分析  

Analysis on respiratory pathogenic spectrum of influenza like cases with negative influenza virus nucleic acid in children under five years old in Baiyin City

作　　者：王彦博[1] 张宾 张慧[3] 张小梅[1] 杨雅倩 WANG Yanbo;ZHANG Bin;ZHANG Hui;ZHANG Xiaomei;YANG Yaqian(Inspection and Testing Department,Baiyin Center for Disease Control and Prevention,Baiyin Gansu,730900,China;School of Public Health,Gansu University of Chinese Medicine,Lanzhou Gansu,730000,China;Pathogen Detection Institute,Gansu Provincial Center for Disease Prevention and Control,Lanzhou Gansu,730000,China)

机构地区：[1]白银市疾病预防控制中心检验检测科,甘肃白银730900 [2]甘肃中医药大学公共卫生学院,甘肃兰州730000 [3]甘肃省疾病预防控制中心病原检测所,甘肃兰州730000

出　　处：《职业与健康》2024年第8期1020-1023,共4页Occupation and Health

基　　金：甘肃省青年科技基金计划(20JR5RA141)。

摘　　要：目的 分析白银市5岁以下儿童流感病毒核酸阴性的流感样病例(influenza-like illness,ILI)鼻咽分泌物的呼吸道病原谱,为儿童呼吸道疾病的防控提供参考依据。方法 对2020年11月—2021年5月409例5岁以下儿童流感病毒核酸阴性的ILI鼻咽分泌物标本采用荧光定量多重PCR技术(TaqMan呼吸道病原微流体芯片)检测人副流感病毒、呼吸道合胞病毒、鼻病毒、肠道病毒、肺炎衣原体、肺炎支原体、流感嗜血杆菌、耶氏肺孢子虫等38种/型呼吸道病原体,并对数据进行分析。结果 409份标本中,未检出本研究所使用的TaqMan呼吸道病原微流体芯片所涵盖的细菌、支原体、衣原体及真菌,病毒的总检出率为60.64%(245/409),其中副流感病毒13.45%(55/409)、呼吸道合胞病毒12.47%(51/409)、鼻病毒9.29%(38/409)、肠道病毒8.80%(36/409)、人偏肺病毒6.60%(27/409)、腺病毒5.62%(23/409)、冠状病毒2.20%(9/409)、博卡病毒2.20%(9/409);患儿病毒总感染率为50.61%,其中单一病毒感染率为41.81%、病毒混合感染率为8.80%,鼻病毒与肠道病毒混合感染最多占38.89%。结论 白银市5岁以下儿童流感病毒核酸阴性的ILI鼻咽分泌物中检出多种呼吸道病毒,病原谱较为复杂,且不同年龄组患儿呼吸道病毒感染阳性检出率存在差异,部分患儿存在≥2种病毒混合感染,应加强儿童ILI的鉴别诊断和监测,保障儿童健康成长。Objective To analyze the respiratory pathogenic spectrum of nasopharyngeal secretions of influenza-like illness(ILI)cases with negative influenza virus nucleic acid in children under five years old in Baiyin City,provide the basis for the prevention and control of respiratory diseases in children.Methods The fluorescence quantitative multiplex PCR technology(TaqMan respiratory pathogenic microfluidic chip) was used to detect 38 respiratory pathogens,including human parainfluenza virus,respiratory syncytial virus,rhinovirus,enterovirus,chlamydia pneumoniae,mycoplasma pneumoniae,Haemophilus influenzae and Pneumocystis pneumoniae,in the nasopharyngeal secretion specimens from 409 ILI cases with negative influenza virus nucleic acid in children under five years old from November 2020 to May 2021,and the data were analyzed.Results Among 409samples,no bacteria,mycoplasma,chlamydia and fungi covered by the TaqMan respiratory pathogenic microfluidic chip of this study were detected.The total detection rate of viruses was 60.64%(245/409),including 13.45%(55/409) of parainfluenza virus,12.47%(51/409) of respiratory syncytial virus,9.29%(38/409) of rhinovirus,8.80%(36/409) of enterovirus,6.60%(27/409) of human metapneumovirus,5.62%(23/409) of adenovirus,2.20%(9/409) of coronavirus,and 2.20%(9/409) of bocavirus.The total virus infection rate of children was 50.61%,of which the single virus infection rate was 41.81%,the mixed virus infection rate was8.80%,and the mixed infection rate of rhinovirus and enterovirus was the highest,which was 38.89%.Conclusion A variety of respiratory viruses are detected in the nasopharyngeal secretions of ILI cases with negative influenza virus nucleic acid in children under five years old in Baiyin City,the pathogenic spectrum is complex,the positive detection rate of respiratory virus infection in children of different age groups is different,some children have mixed infection of two or more viruses,so the differential diagnosis and monitoring of children's ILI should be strengthened to ensur

关 键 词：流感病毒核酸阴性 流感样病例 呼吸道病原谱 混合感染 

分 类 号：R446[医药卫生—诊断学]