夏季过夜滞留诱导室内管道饮用水细菌增殖特征  

Characteristics of Bacterial Growth in Indoor Drinking Water Pipe Induced by Summer Overnight Stagnation

作　　者：张海涵 刘祥 徐磊 黄廷林[1,2] ZHANG Hai‑han;LIU Xiang;XU Lei;HUANG Ting‑lin(Shaanxi Key Laboratory of Environmental Engineering,School of Environmental and Municipal Engineering,Xi’an University of Architecture and Technology,Xi’an 710055,China;Northwest China Key Laboratory of Water Resource and Environment Ecology,Xi’an 710055,China)

机构地区：[1]西安建筑科技大学环境与市政工程学院陕西省环境工程重点实验室,陕西西安710055 [2]西北水资源与环境生态教育部重点实验室,陕西西安710055

出　　处：《中国给水排水》2024年第11期57-64,共8页China Water & Wastewater

基　　金：国家自然科学基金资助项目(51978561、51979217);陕西省重点研发创新链项目(2019ZDLSF06-03);陕西高校青年创新团队项目(21JP061)。

摘　　要：针对室内饮用水管道过夜滞留水水质的恶化问题,开展了系统性研究。6月—8月期间,每月对室内饮用水管道新鲜水与滞留水水质进行检测,采用流式细胞仪与三磷酸腺苷(ATP)对细菌数量及细菌活性进行分析。结果表明,过夜滞留饮用水安全风险加剧,余氯浓度显著下降。6月、7月、8月滞留水NO2--N浓度范围分别为0~0.006、0~0.009和0~0.012 mg/L。相较于新鲜水,滞留水6月、7月、8月Fe浓度分别增加1.33、1.63和6.00倍。经过滞留之后,总细菌数和ATP浓度显著增加,滞留水总细菌数分别为1.41×10^(5)、1.23×105和2.82×105cells/mL,细菌总ATP浓度和胞内ATP浓度分别为7.97×10^(-12)、3.91×10^(-12)、12.80×10^(-12)g/mL和7.15×10^(-17)、4.74×10^(-17)、4.48×10^(-17)g/cell。结构方程模型分析表明,在滞留水和新鲜水中,温度对细菌数量与活性均起到显著正相关作用。滞留之后Fe对细菌数量的相关性由负相关转变为正相关,而细菌数量对细菌活性的正相关作用减弱。This paper systematically investigated the deterioration of overnight water quality in indoor drinking water pipe.From June to August,the monthly testing of fresh and stagnant water in indoor drinking water pipe was conducted,and the cell count and biological activity of bacteria were analyzed using flow cytometry and adenosine triphosphate(ATP).The risk of drinking water safety escalated after overnight stagnation.The residual chlorine in drinking water decreased significantly following stagnation.In June,July,and August,the nitrite nitrogen ranged from 0 mg/L to 0.006 mg/L,0 mg/L to 0.009 mg/L,and 0 mg/L to 0.012 mg/L,respectively.In comparison to fresh water,the concentration of Fe in stagnant water increased by 1.33 times,1.63 times and 6.00 times in June,July,and August respectively.Following stagnation,there was a significant increase in cell count and ATP concentration.The total bacterial cell counts in stagnant water were 1.41V_(254)cells/mL,1.23V_(254)cells/mL and 2.82V_(254)cells/mL,respectively.The total ATP concentration of bacteria and their intracellular ATP concentration were 7.97×10^(-12)g/mL,3.91×10^(-12)g/mL,12.80×10^(-12)g/mL and 7.15×10^(-17)g/cell,4.74×10^(-17)g/cell,4.48×10^(-17)g/cell,respectively.The structural equation model analysis revealed a significant positive correlation between temperature and the abundance and activity of bacteria in both stagnant water and fresh water.After stagnation,the relationship between Fe and bacterial cell counts shifted from negative to positive,while the positive correlation between bacterial cell counts and bacterial activity was attenuated.

关 键 词：室内饮用水管道 过夜滞留 细菌增殖 代谢活性 结构方程 

分 类 号：TU991[建筑科学—市政工程]