离体悬浮生物膜特性的演变规律  

Evolution of Isolated Suspended Biofilm Properties

作　　者：胡小兵[1,2] 宋维维 王振振 沈翼军[1,2] 刘浩宇 李晶晶 汪坤 HU Xiao-bing;SONG Wei-wei;WANG Zhen-zhen;SHEN Yi-jun;LIU Hao-yu;LI Jing-jing;WANG Kun(College of Architectural Engineering,Anhui University of Technology,Ma’anshan 243002,China;Engineering Research Center of Biofilm Water Purification and Utilization Technology,Ma’anshan 243032,China)

机构地区：[1]安徽工业大学建筑工程学院,安徽马鞍山243002 [2]生物膜法水质净化及利用技术教育部工程研究中心,安徽马鞍山243032

出　　处：《中国给水排水》2024年第11期95-102,共8页China Water & Wastewater

基　　金：生物膜法水质净化及利用技术教育部工程研究中心项目(BWPU2021ZY01,BRT19-02);安徽省重点研究与开发计划项目(202004h07020027)。

摘　　要：为探索生物膜演变规律,将载体表面成熟生物膜剥离置于反应器中培养35 d,分别从絮体微观形态结构(当量直径D_(eq)、孔隙率P_(o)、规则度Re、长径比AR)、生物膜理化特征(生物量MLSS、胞外聚合物EPS、SVI)和污水处理性能三个方面考察纯培养离体悬浮生物膜的形态演变特征。结果表明,随着培养时间的延长,生物膜发生裂解变成较多的小絮体,其当量直径不断降低,孔隙率、规则度波动上升,长径比变化不明显。生物膜的生物量整体呈上升趋势,培养后期,MLSS、MLVSS及MLVSS/MLSS(f)分别达到最大值7.68 mg/mL、6.91 mg/mL和0.92。SVI呈波动上升趋势。EPS呈“先减后增”的变化趋势,培养后期总EPS(Tol-EPS)达到98.19 mg/g。运行后期,对COD、NH_(4)^(+)-N、TP的平均去除率分别达到(81.98±2.51)%、(89.86±13.67)%和(85.81±9.57)%。总之,离体悬浮生物膜培养后期,其性能指标与活性污泥接近,生物膜演变为活性污泥。The mature biofilm on the carrier surface was stripped and cultured in a reactor for 35 days to investigate the evolution of biofilm into activated sludge.The morphological characteristics of pure cultured suspended biofilm were investigated from three aspects:floc microstructure(equivalent diameter,porosity,regularity and aspect ratio),physicochemical properties of biofilm(MLSS,EPS and SVI),and wastewater treatment performance.With the extension of culture time,the biofilm disintegrated into more flocs,leading to a decrease in its equivalent diameter,fluctuation up in porosity and regularity,while the aspect ratio remained relatively unchanged.The biomass concentration of the biofilm exhibited an overall upward trend,with MLSS,MLVSS,and MLVSS/MLSS(f)peaking at 7.68 mg/mL,6.91 mg/mL,and 0.92 respectively during the later stage of cultivation.The SVI demonstrated a fluctuating upward trajectory.The EPS exhibited a trend of initial decrease followed by an increase,with total EPS(Tol⁃EPS)reaching 98.19 mg/g in the late stage of cultivation.The average removal rates of COD,NH_(4)^(+)-N and TP reached(81.98±2.51)%,(89.86±13.67)%and(85.81±9.57)%,respectively.In conclusion,during the later stage of suspended biofilm culture,its performance indicators closely resembled those of activated sludge,indicating the evolution of the biofilm into activated sludge.

关 键 词：悬浮生物膜 演变 絮体结构 胞外聚合物(EPS) 

分 类 号：TU992[建筑科学—市政工程]