高粱RKD基因家族鉴定及氮胁迫下的表达模式分析  

Identification of Sorghum RKD Gene Family and Its Expression Patterns Analysis under Nitrogen Stress

作　　者：曲匡正 李丹[1] 陆晓春[1] 朱振兴[1] Qu Kuangzheng;Li Dan;Lu Xiaochun;Zhu Zhenxing(Sorghum Research Institute,Liaoning Academy of Agricultural Sciences,Shenyang,110161)

机构地区：[1]辽宁省农业科学院高粱研究所,沈阳110161

出　　处：《分子植物育种》2024年第12期3842-3850,共9页Molecular Plant Breeding

基　　金：辽宁省优秀青年自然基金项目(2021-YQ-04);中国博士后面上基金项目(2021M693848);国家现代农业产业技术体系建设项目(CARS-06-14.5-A3)共同资助。

摘　　要：RKD(RWP-RK domain protein)是植物中特有的氮响应相关蛋白,在植物生长发育,尤其是生殖过程中起到重要作用。高粱(Sorghum bicolor)是一种耐瘠薄、抗旱的粮食作物,挖掘其养分响应与利用相关基因,对于解析高粱养分响应的分子遗传机制,培育养分高效作物具有重要意义。本研究通过生物信息学的方法,鉴定到分布在5条染色体上的7个高粱RKD基因。对SbRKD蛋白的理化性质和保守结构域进行分析,发现氨基酸残基数目对于蛋白性质影响较大。对高粱、水稻和拟南芥的RKD基因进行进化分析发现其主要聚成三类,且SbRKDs都受到纯化选择压力,同时有部分SbRKD基因在物种分化过程中发生缺失和复制。SbRKD基因组织表达结果说明多数RKD基因在高粱中表达水平低,只有SbRKD1和SbRKD4在种子和胚中的表达水平较高。氮饥饿和高氮诱导结果表明,SbRKD1和SbRKD4能够响应外源氮元素供应。本研究为后续探索RKD基因对高粱生长发育的影响提供了理论依据。RKD(RWP-RK domain protein)is a specific nitrogen responsive protein in plants,which plays important roles in plant growth,especially in reproductive processes.Sorghum bicolor is a grain crop with resistant to drought and barren.Exploring genes related to nutrient uptake and utilization is vital for analyzing molecular mechanism of sorghum nutrient response and for cultivating nutrient efficient crops.In this study,7 sorghum RKD genes distributed on 5 chromosomes using bioinformatics methods.Analysis of protein properties and conserved domains of SbRKD revealed that the number of amino acid residues had a significant impact on their properties.Evolutionary analysis of RKD genes in sorghum,rice,and Arabidopsis revealed that they mainly aggregated into three clades and SbRKDs were all under purifying selection.Meanwhile,some SbRKD genes occurred deletion and replication during differentiation.Most RKD genes had low expression levels in sorghum due to tissue expression results,except for SbRKD1 and SbRKD4,which had high expression levels in embryos and seeds,respectively.The results of nitrogen starvation and high concentration nitrogen induction indicated that SbRKD1 and SbRKD4 could respond to external supplement of nitrogen.The study provided a theoretical basis for future exploring the impact of RKD genes on sorghum growth and development.

关 键 词：高粱 RKD基因 氮饥饿 氮诱导 

分 类 号：S514[农业科学—作物学]