中间锦鸡儿CiS40-11基因的克隆及表达模式分析  

Cloning and Expression Pattern Analysis of CiS40-11 Gene from Caragana intermedia

作　　者：张敏娜 杨天瑞[1] 李会园 雷艳红 李国婧[1] 王瑞刚[1] Zhang Minna;Yang Tianrui;Li Huiyuan;Lei Yanhong;Li Guojing;Wang Ruigang(Key Laboratory of Plant Stress Physiology and Molecular Biology,College of Life Science,Inner Mongolia Agricultural University,Hohhot,010018;College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot,010018;Agriculture and Animal Husbandry Bureau,Chifeng,024500)

机构地区：[1]内蒙古农业大学生命科学学院,植物逆境生理与分子生物学自治区重点实验室,呼和浩特010018 [2]内蒙古农业大学园艺与植保学院,呼和浩特010018 [3]内蒙古赤峰市翁牛特旗农牧局,赤峰024500

出　　处：《分子植物育种》2024年第12期3905-3911,共7页Molecular Plant Breeding

基　　金：内蒙古自治区自然科学基金项目(2019ZD05);内蒙古自治区科技计划项目(2019GG007);内蒙古自治区研究生教育创新计划项目(SZ2020087)共同资助。

摘　　要：CiS40-11基因为中间锦鸡儿SnR基因家族的成员,为研究其在非生物胁迫中的功能,对CiS40-11基因ORF序列进行克隆,并进行组织特异性和非生物胁迫下表达模式的分析。结果表明,CiS40-11基因ORF长633 bp,编码了一个由210个氨基酸组成的亲水性蛋白,其大小为23.06 kD,等电点为5.70。组织特异性分析表明CiS40-11在叶中的表达水平最高;非生物胁迫表达模式分析结果显示CiS40-11在冷和干旱胁迫的诱导下,表达水平会持续性的降低。本研究为进一步揭示CiS40-11在植物非生物胁迫调控过程中的功能及作用机理提供了初步基础。CiS40-11 is a member of SnR family from Caragana intermedia.In order to understand its function in abiotic stress,the ORF of CiS40-11 was cloned,and its tissue specificity and abiotic stress expression pattern was analyzed.The ORF of CiS40-11 is 633 bp,CiS40-11 composed of 210 amino acids,its molecular weight is about 23.06 kD and the isoelectric point is 5.70.Tissue-specific analysis shows that the maximum expression of CiS40-11 is showed in leaves.In addition,the expression of CiS40-11 continuously decreased under cold and drought stress treatment.This study provides a theoretical basis for further functional analysis and regulation mechanism of CiS40-11 in the process of abiotic stress.

关 键 词：CiS40-11 中间锦鸡儿 基因克隆 非生物胁迫 表达分析 

分 类 号：S793.3[农业科学—林木遗传育种]