湿加松松针16种黄酮含量变异分析及SNP位点挖掘  

Variation Analysis and SNP Locus Mining of 16 Flavonoids Content in Needle of Pinus elliotti×Pinus caribaea

作　　者：李福明 黄婷 钟岁英 谭志强 邓乐平 张智霖 林昌明 毛积鹏 Li Fuming;Huang Ting;Zhong Suiying;Tan Zhiqiang;Deng Leping;Zhang Zhilin;Lin Changming;Mao Jipeng(Taishan Hongling Seed Orchart,Taishan,529223;Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm,South China Agricultural University,Guangzhou,510642)

机构地区：[1]台山市红岭种子园,台山529223 [2]华南农业大学,广东省森林植物种质创新与利用重点实验室,广州510642

出　　处：《分子植物育种》2024年第12期3939-3946,共8页Molecular Plant Breeding

基　　金：中央林业改革发展资金项目(粤财资环[2019]37号)资助。

摘　　要：为了研究黄酮类物质在湿加松松针组织中的含量变异特性及挖掘与黄酮含量显著相关的单核苷酸多态性(single nucleotide polymorphism,SNP)位点。以120个湿加松针叶样本为研究材料,进行了16种黄酮化合物的含量测定和转录组测序(RNA-seq)分析。结果发现,16种黄酮化合物在湿加松不同样本间的含量差异显著,变异系数介于28.21%~80.05%之间。RNA-seq分析共获得307202329个高质量reads,拼接组装后得到98673个unigenes,其中32689个unigenes获得功能注释信息。鉴定到373个和黄酮类化合物生物合成相关的unigenes。通过比对RNA-seq结果,共发现378549个SNP位点,平均SNP频率为109 bp/SNP。并鉴定到47个和黄酮含量显著相关的SNP位点。本研究为高黄酮含量的湿加松良种选育和分子标记辅助育种工作提供了理论基础。In order to study the content variation characteristics of flavonoids in needle of P.elliotti×P.caribaea,and mining the single nucleotide polymorphism(SNP)significantly related to the content of flavonoids.The content of 16 flavonoids were determined and RNA-seq analysis was performed in 120 samples of P.elliotti× P.caribaea.The results showed that the content of 16 flavonoids varied significantly among different samples,and the coefficient of variation ranged from 28.21%to 80.05%.A total of 307202329 high-quality reads were obtained,and assembled into 98673 unigenes.Among which,32689 unigenes were obtained with functional annotation.In addition,373 unigenes related to the biosynthesis of flavonoid were identified.A total of 378549 SNPs were found by blast the RNA-seq to reference genome,with an average SNP frequency of 109 bp/SNP.A total of 47 SNPs were identified that were significantly related to flavonoids content.This study provides an theoretical basis for the breeding and molecular marker-assisted breeding of P.elliotti×P.caribaea with high flavonoids content.

关 键 词：湿加松 黄酮化合物 转录组测序 SNPS 

分 类 号：S791.24[农业科学—林木遗传育种]