黄籽甘蓝型油菜的合成和不同颜色种皮比较代谢组分析  

Resynthesis of Yellow-seeded Brassica nap us and Comparative Metabonomic Analysis of Differently Colored Seed Coats

作　　者：杨芳杰 叶沈华 马晓伟 陈延平 易斌 马朝芝 沈金雄[1] 涂金星[1] 傅廷栋[1] 文静[1] Yang Fangjie;Ye Shenhua;Ma Xiaowei;Chen Yanping;Yi Bin;Ma Chaozhi;Shen Jinxiong;Tu Jinxing;Fu Tingdong;Wen Jing(College of Plant Science and Technology of Huazhong Agricultural University,National Research Center of Rapeseed Engineering and Technology,Wuhan,430070)

机构地区：[1]华中农业大学植物科学技术学院,国家油菜工程技术研究中心,武汉430070

出　　处：《分子植物育种》2024年第12期3979-3987,共9页Molecular Plant Breeding

基　　金：国家重点研发计划项目(2017YFD0101702);财政部、农业农村部:国家现代农业产业技术体系项目(CARS-12)共同资助。

摘　　要：为分析人工合成甘蓝型油菜的稳定性以及黄黑籽种皮中的代谢物差异,本研究以一个A_(4)代甘蓝型油菜人工合成种为材料,调查其细胞学稳定性,对其自交A10代黄、黑籽株系进行了种皮香草醛染色和广泛靶向代谢组分析,结果表明人工合成甘蓝型油菜A_(4)代株系具有稳定的38条染色体,有丝分裂和减数分裂行为正常。A_(10)代黄籽和黑籽株系种皮颜色差异与原花青素差异积累有关,黑籽材料从开花后30 d开始,原花青素逐步在种皮中积累,而黄籽种皮中几乎没有原花青素积累。授粉后50 d的黄、黑籽种皮中共检测出23类737种代谢物,其中差异代谢物343种。与黑籽种皮相比,黄籽种皮中分别有149种和194种代谢物显著上调和下调积累。差异代谢物显著富集在黄酮和黄酮醇生物合成路径、甘油酯代谢路径和花青素生物合成途径这3条代谢通路中。综上所述,该人工合成甘蓝型油菜黄籽和黑籽种皮颜色差异主要由原花青素等类黄酮物质的差异积累导致。研究结果为揭示甘蓝型油菜种皮颜色形成机理奠定了理论基础,为黄籽油菜品种选育提供了材料支持。To study the stability of resynthesized Brassica napus and the differences of accumulated metabolites between the yellow and black seed coats of resynthesized B.napus,the cytological stability of an A_(4) line of resynthesized B.napus was investigated.Then vanillin staining and widely targeted metabolomics were conducted in seed coats of yellow and black seeded A_(10) lines which derived from self-pollination of the A_(4) line.The results showed that the A,line exhibited normal mitotic and meiotic behaviors with a somatic chromosome number of 38.The color difference of the seed coats in these two A_(10) lines related with the accumulation of proanthocyanidins.The accumulation of proanthocyanidins in black-seeded coats were increased gradually after 30 days after flowering(DAF),whereas little proanthocyanidins were detected in yellow-seeded coats.A total of 737 metabolites from 23 classes were detected in the yellow and black seed coats at 50 DAF.A sum of 343 differentially accumulated metabolites were identified.Compared with black seed coats,149 and 194 metabolites accumulated significantly higher and lower in yellow seed coats,respectively.The differentially accumulated metabolites are mainly enriched in three metabolic pathways,including flavone and flavonol biosynthesis,glycerolipid metabolism and anthocyanin biosynthesis.In summary,the different seed coat color in this resynthesized B.napus attributes to the different accumulation of proanthocyanidins and other flavonoid metabolites in the seed coats.The results laid a theoretical foundation for revealing the mechanism of seed coat color formation,and provided new germplasm for breeding yellow-seeded B.napus varieties.

关 键 词：甘蓝型油菜人工合成种 黄籽 代谢组学 原花青素 种皮颜色 

分 类 号：S565.4[农业科学—作物学]