石碌含笑贮藏花粉的萌发特性与花粉活力比较  

Germination Characteristics and Pollen Viability Comparison of Michelia shiluens is(Magnoliaceae)Stored Pollen

作　　者：莫丽文 孟新亚 宋希强[1,2] 赵莹[1,2] 王亚玲 Mo Liwen;Meng Xinya;Song Xiqiang;Zhao Ying;Wang Yaling(Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants(Ministry of Education),College of Forestry,Hainan University,Haikou,570228;Key Laboratory of Biology of Flower Resources of Hainan Province,College of Forestry,Hainan University,Haikou,570228)

机构地区：[1]海南大学林学院,热带特色林木花卉遗传与种质创新教育部重点实验室,海口570228 [2]海南大学林学院,海南省热带特色花木资源生物学重点实验室,海口570228

出　　处：《分子植物育种》2024年第12期3994-4000,共7页Molecular Plant Breeding

基　　金：海南省重点研发计划项目(ZDYF2020099);海南国家公园研究院科研课题(KY-22ZK08)共同资助。

摘　　要：本研究以石碌含笑(Michelia shiluensis)花粉为试验材料,研究不同浓度梯度下蔗糖(50~150 g/L)、硼酸(100~300 mg/L)、氯化钙(200~400 mg/L)对花粉离体萌发的影响,以及不同贮藏温度(25℃, 4℃,-20℃,-80℃)下花粉离体萌发率和染色率的变化。研究结果表明:离体萌发最适培养基为蔗糖50 g/L、硼酸100 mg/L、氯化钙400 mg/L,其对石碌含笑离体花粉萌发率重要性的影响依次为硼酸>蔗糖>氯化钙;干燥条件下,-80℃环境更有利于石碌含笑花粉的保存;MTT染色法最适合石碌含笑花粉活力的快速鉴定,醋酸洋红染色法不适于石碌含笑花粉活力测定。研究结果可为石碌含笑的花粉收集、贮藏和活力检测提供科学依据和实践指导。tIn this research,with pollens of Michelia shiluensis as test material,different concentrations of sucrose(50~150 g/L),H,BO;(100~300 mg/L)and CaCl_(2)(200~400 mg/L)on pollen germination in vitro of Michelia shiluensis were observed.Moreover,the changes of pollen germination in vitro and stain rates of Michelia shiluensis at different storage temperatures(25℃,4 ℃,-20 ℃,-80℃ )were studied.The results showed that:the medium with 50 g/L sucrose+100 mg/L H_(3)BO_(3)+400 mg/L CaCl_(2) was the most suitable for germination in vitro,and the importance of Michelia shiluensis pollen germination rate in vitro were H_(3)BO_(3)>sucrose>CaCl_(2) in order.Under dry conditions,-20℃ and-80 ℃,were more conducive to the preservation of Michelia shiluensis pollen.The MTT stain method was the most suitable for the rapid identification of the pollen activity of Michelia shiluensis and the magenta acetate stain method was not suitable for the determination of the pollen activity of Michelia shiluensis.This study can provide scientific and practical guidance for pollen collection,storage and vitality detectionof Micheliashiluensis.

关 键 词：石碌含笑(Michelia shiluensis) 离体花粉 花粉贮藏 花粉活性检测 木兰科 

分 类 号：S685.99[农业科学—观赏园艺]