蔓性千斤拔丛生芽诱导及离体快繁技术建立  

Induction of Cluster Buds and Establishment of Rapid Propagation Technology in vitro of Flemingia philippinensis Merr.et Rolfe

作　　者：李娜 徐丹 陈汶钊 刘幽言 黄瑞华 杜勤[1] Li Na;Xu Dan;Chen Wenzhao;Liu Youyan;Huang Ruihuaa;Du Qin(College of Traditional Chinese Medicine,Guangzhou University of Chinese Medicine,Guangzhou,510006)

机构地区：[1]广州中医药大学中药学院,广州510006

出　　处：《分子植物育种》2024年第12期4001-4008,共8页Molecular Plant Breeding

基　　金：2019年医疗服务与保障能力提升补助资金(中医药事业传承与发展部分)“全国中药资源普查项目”(财社[2019]39号);广州中医药大学大学生创新创业训练计划项目(202010572239)共同资助。

摘　　要：为探究蔓性千斤拔(Flemingia philippinensis Merr.et Rolfe)离体培养过程中各生长阶段最佳的激素组合,筛选适宜的外植体及植株再生途径,建立其快速繁殖体系。本研究以蔓性千斤拔无菌实生苗带腋芽的茎段、茎尖及叶片为材料,通过完全组合试验研究不同植物激素及其质量浓度对愈伤组织诱导、腋芽萌发、基茎不定丛芽诱导及植株再生的影响。结果表明:蔓性千斤拔增殖方式以直接器官发生为主,带腋芽的茎段在MS+6-BA 0.5 mg/L+NAA 0.2 mg/L+AC 1.0 g/L中培养60 d后,基部生长大量不定丛生芽,增殖系数达9.85;生根的适宜培养基为MS+NAA 0.5 mg/L,培养45 d后生根率为100%;再生植株经炼苗移栽至配比为泥炭土∶腐殖土∶珍珠岩∶蛭石=4∶4∶1∶1的基质中,成活率达86%。综上已筛选出的条件,本研究成功建立了基部不定丛生芽诱导、植株生根效果好及再生植株成活率高的蔓性千斤拔离体快繁技术,为保护其野生资源、优质种苗繁育提供了有效途径。In order to optimize the best hormone conditions for each growth stage during the in vitro culture of Flemingia philippinensis Merr.et Rolfe,to select the suitable explants and plant regeneration methods to establish a rapid propagation system.In this study,the stem segments,stem tips,and leaves of the vine-like aseptic seedlings with axillary buds were used as materials.The effects of different plant hormones and their concentrations on callus induction,axillary bud germination,basal stem adventitious bud induction,and plant regeneration were studied through a complete combination experiment.The results showed that the proliferation mode of F.philippinensis was mainly direct organogenesis,and the stems with axillary buds were cultured in MS+6-BA 0.5 mg/L+NAA 0.2 mg/L+AC 1 g/L for 60 days.A large number of adventitious cluster buds grow,and the valueadded coefficient reaches 9.85;the suitable medium for rooting was MS+NAA 0.5 mg/L,the strong plant regeneration occurred 45 days later with the rooting rate up to 100%;the regenerated plants were transplanted to a matrix of peat soil:humus soil:perlite:vermiculite=4:4:1:1,and the survival rate reached 86%.In summary of the screened conditions,this study successfully established a fast propagation technique for the in vitro propagation of F.philippinensis with good basal adventitious shoot induction and plant rooting effect and high survival rate of regenerated plants,which provides an effective way to protect its wild resources and breed high-quality seedlings.

关 键 词：蔓性千斤拔 离体快繁 基茎不定丛生芽 褐化 植株再生 

分 类 号：S567.19[农业科学—中草药栽培]