一种烟草根结线虫病害抗性复合评价标准的建立  

Establishment of a Composite Evaluation Standard for Tobacco Root Knot Nematode Disease Resistance

作　　者：周绍芳 高泽文[1] 李红 吴文涛 焦芳婵[2] 王扬[1] Zhou Shaofang;Gao Zewen;Li Hong;Wu Wentao;Jiao Fangchan;Wang Yang(College of Plant Protection,Yunnan Agricultural University,Kunming,650201;Yunnan Academy of Tobacco Agricultural Science,Kunming,650201)

机构地区：[1]云南农业大学植物保护学院,昆明650201 [2]云南省烟草农业科学研究院,昆明650201

出　　处：《分子植物育种》2024年第12期4051-4058,共8页Molecular Plant Breeding

基　　金：中国烟草总公司云南省公司项目(2019530000241001)资助。

摘　　要：烟草根结线虫病害是一种重要病害,为建立一个生理和病理的抗性复合评价标准以筛选出具有耐病性的烟草种质植株。本研究在云南的5个主要烟草种植县进行了调查,在烟草采收期系统地调查了发病烟株的根结百分率、根褐变比例、最大根结直径、叶尖枯焦以及脚叶部茎围等与病害相关的病理及生理指标,对数据进行统计和相关性分析,拟定出烟草根结线虫病抗性值评价计算公式:抗性值=根结百分率×50+根褐变比例×30+最大根结直径赋予值×20;根据发病程度建立的抗性标准为:免疫:0;高抗:1~15;中抗:16~30;抗:31~50;感:51~80;高感:81~100。对温室盆栽接种根结线虫60 d的烟草单株进行了抗性复合评价,结果表明烟草单株表现出不同的抗感性,其中40株为感病,20株抗病。本研究建立的抗性复合评价标准可以为选育出具有抗烟草根结线虫的烟草品种奠定基础,且与原有的抗性评价标准相比,也能够更为细致的划分出有耐病潜力的单株。Tobacco root knot nematode is an important disease to establish a physiological and pathological resistance composite evaluation standard,and screen tobacco germplasm plants with disease resistance.This study conducted an investigation in five major tobacco planting counties in Yunnan.During the tobacco harvest period,the pathological and physiological indexes related to the disease,such as the percentage of root knot,the proportion of root browning,the maximum root knot diameter,the withered leaf tip and the stem circumference of foot leaf,were systematically investigated.The data were statistically and correlated,and the evaluation and calculation formula of tobacco root knot nematode disease was worked out:Value of resistance=root knot percentagex50+root browning ratiox30+assigned value of maximum root knot diameterx20;The results are as follows immune:0;high resistance:1~15;moderate resistance:16~30;resistance:31~50;susceptibility:51~80;high susceptibility:81~100.On this basis,the resistance of tobacco plants inoculated with root knot nematode in greenhouse for 60 days was evaluated and identified.The results showed that tobacco plants showed different resistance,of which 40 were susceptible and 20 were resistant.The composite evaluation criteria established in this study can lay a foundation for the selection of tobacco varieties with resistance to tobacco root knot nematode,and compared with the original resistance evaluation criteria,the individual plants with disease resistance potential can be divided more carefully.

关 键 词：根结线虫病害 抗性选育 抗性复合评价 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]