响应曲面法优化梅花鹿茸多糖提取工艺  

Optimization of Extraction Process of Polysaccharides from Sika Deer Antler by Response Surface Methodology

作　　者：葛安璐 谭世媛 鞠美琪 庞春颖[1] 张淑华[1] GE Anu;TAN Shiyuan;JU Meiqi;PANG Chunying;ZHANG Shuhua(School of Life Science and Technology,Changchun University of Science and Technology,Changchun 130022)

机构地区：[1]长春理工大学生命科学技术学院,长春130022

出　　处：《长春理工大学学报（自然科学版）》2024年第3期84-92,共9页Journal of Changchun University of Science and Technology(Natural Science Edition)

基　　金：吉林省科技厅科技发展计划项目(20220204127YY)。

摘　　要：以梅花鹿茸多糖为研究对象,首次采用超声波提取法、热水浸提法、水提醇沉法相结合的方式优化提取工艺。单因素实验基础上,利用BBD实验设计原理进行四因素三水平的响应面实验。苯酚硫酸法测定多糖含量,紫外光谱和FT-IR光谱分析检测纯度。结果表明:最佳提取工艺条件下,超声液料比34∶1、超声时间43 min、浸提温度54℃、浸提时间2 h,多糖提取率达2.02%,多糖含量70.05%,不含核酸和蛋白质等成分并具有典型多糖结构,表明优化的梅花鹿茸多糖提取工艺切实可行,优化结果优于先前工艺。Taking Sika Deer Polysaccharide as the research object,the extraction process was optimized for the first time by the combination of ultrasonic extraction,hot water leaching and aqueous alcohol precipitation.On the basis of the one-factor test,a four-factor,three-level response surface test was carried out using the principle of BBD experimental design.The polysaccharide content was determined by phenol sulfuric acid method,and the purity was detected by UV and FT-IR spectral analysis.The results showed that under the optimal extraction process conditions:ultrasonic liquid to material ratio of 34∶1,ultrasonic time of 43 min,extraction temperature of 54℃,and extraction time of 2 h,the polysaccharide extraction rate reached 2.02%,and the polysaccharide content of 70.05%,which was free of nucleic acids and proteins,etc.,and had a typical polysaccharide structure,which optimised process for extracting Sika Deer Antler Polysaccharide was shown to be practical and feasible,and the results were better than those of the previous process.

关 键 词：梅花鹿茸多糖 响应曲面法 苯酚硫酸法 FT-IR光谱分析 

分 类 号：O482.31[理学—固体物理]