铜绿假单胞菌厌氧胁迫丙酮酸发酵维持生存相关基因研究  

Long-term Anaerobic Survival Related Genes of Pseudomonas Aeruginosa Under Anaerobic Pyruvate Fermentation

作　　者：于璇 安起弘 史翔予 金秀妍 张昊[1] YU Xuan;AN Qihong;SHI Xiangyu;JIN Xiuyan;ZHANG Hao(School of Life Science and Technology,Changchun University of Science and Technology,Changchun 130022)

机构地区：[1]长春理工大学生命科学技术学院,长春130022

出　　处：《长春理工大学学报（自然科学版）》2024年第3期108-115,共8页Journal of Changchun University of Science and Technology(Natural Science Edition)

基　　金：吉林省科技发展计划项目(20220402038GH,20210101025JC)。

摘　　要：为深入研究铜绿假单胞菌响应厌氧丙酮酸发酵维持长期存活相关基因,采用同源重组蔗糖反向筛选构建P. aeruginosa PAO1广谱胁迫蛋白uspK敲除株ΔPA3309,在厌氧丙酮酸发酵条件下检测野生株与敲除株表型与基因型差异。细菌存活曲线、NADH/NADPH表型差异,表明敲除株在该条件下处于高能状态、代谢速率快、存活期短;转录组差异进一步印证野生株能量代谢、信号传导基因转录低于突变株,其TetR、LysR转录调节因子下调接近220,SDR氧化还原酶下调超过215,表明野生株可能通过UspK蛋白作用转录因子,进而下调氧化还原酶等能量代谢基因的转录与表达,控制细菌适应外部厌氧胁迫环境。In order to further study the related functional genes for Pseudomonas aeruginosa in response to anaerobic stress environment and maintaining long-term survival through pyruvate fermentation,homologous recombination sucrose reverse screening technology was used to construct universal stress protein uspK gene knockout mutant strainΔPA3309,and detected the phenotype and genotype differences between the wild type strain PAO1 and the mutant strain under anaerobic pyruvate fermentation condition.The phenotype differences of bacteria survival curves and NADH/NADPH amount showed that the mutant strain was still in a high energy state under anaerobic stress conditions than PAO1.In additional,the genotype differences of transcriptomic data showed that energy metabolism genes and signaling related genes of PAO1 strain were significantly lower than mutant strain.Compared with mutant strain,the TetR and LysR family transcription regulator were down regulated nearly 220,and the SDR family oxidation reductase was down regulated more than 215 in PAO1,indicating that PAO1 may regulate TetR and LysR transcription factors through the UspK protein,further down regulating energy metabolism proteins such as SDR family oxidation reductase under anaerobic stress pyruvate fermentation conditions.

关 键 词：铜绿假单胞菌 厌氧丙酮酸发酵 UspK 基因敲除 转录组 

分 类 号：Q819[生物学—生物工程]