基于细胞黏附的不同微结构3D打印多孔生物支架流体力学有限元分析  

Fluid mechanics finite element analysis of 3D printed porous biological scaffolds with different microstructures based on cell adhesion

作　　者：吴其右 崔博宇 夏炜 刘亦洪[1] WU Qiyou;CUI Boyu;XIA Wei;LIU Yihong(General Department,Peking University Hospital of Stomatology,Beijing 100081,China;Department of Materials Science and Engineering,Uppsala University,Uppsala 75121,Sweden)

机构地区：[1]北京大学口腔医院综合科,北京市100081 [2]瑞典乌普萨拉大学材料科学与工程系,瑞典乌普萨拉75121

出　　处：《组织工程与重建外科》2024年第3期293-299,共7页Journal of Tissue Engineering and Reconstructive Surgery

基　　金：国家自然科学基金(52111530189)。

摘　　要：目的研究不同孔形态结构的3D打印支架应用于骨再生时对细胞黏附的影响。方法利用MSLattice软件设计四种具有不同孔形态的支架结构,分别是立方形(Cubic)、六边形(Hexagon)、钻石形(Diamond)和螺旋形(Gyroid)。利用有限元分析(Finite element method,FEM)和计算机流体建模(Computational fluid dynamics,CFD)的原理,使用Simenns Star CCM+软件对四种支架内部流体域进行组织液流动模拟,模拟细胞黏附过程,测量支架内组织液流速和压力、组织液的渗透率和黏附层厚度。结果当液体流经四种支架后,压力会被逐渐削弱,但由于支架相对规则,压力均呈现出梯度分布,组织液流经四种支架前的压力值模拟按顺序依次为:Gyroid>Cubic>Diamond>Hexagon。流速:Cubic>Gyroid>Diamond>Hexagon。四种支架的渗透率:Diamond>Hexagon>Cubic>Gyroid。黏附层厚度:Gyroid>Hexagon>Cubic>Diamond。结论流体力学分析结果显示,Gyroid支架具有更低的渗透率和最高的黏附层厚度,更有利于细胞黏附。Objective To study the influence of 3D-printed bone scaffolds with different pore morphologies on cell adhesion.Methods Utilizing MSLattice software,four porous structures with different pore morphologies,namely cubic,Hexagon,diamond,and gyroid,were designed.The principles of finite element analysis(FEM)and computational fluid dynamics(CFD)were employed using Siemens Star CCM+software to simulate the fluid flow within the four scaffold structures,model the process of cell adhesion,measure the fluid flow velocity and pressure within the scaffold,as well as the fluid permeability and the thickness of the adhesion layer.Results Upon the passage of fluid through the four types of scaffolds,the pressure gradually diminished.However,due to the relatively regular structure of the scaffolds,the pressure exhibited a gradient distribution.The simulated pressure values before fluid flow through the four types of scaffolds were,in sequence:Gyroid>Cubic>Diamond>Hexagon.Flow velocity:Cubic>Gyroid>Diamond>Hexagon.The permeability of the four types of scaffolds:Diamond>Hexagon>Cubic>Gyroid.Adhesion layer thickness:Gyroid>Hexagon>Cubic>Diamond.Conclusion The fluid mechanics analysis results show that the gyroid scaffold has lower permeability and the highest adhesion layer thickness,which is more conducive to cell adhesion.

关 键 词：有限元 微结构 3D打印 生物支架 细胞黏附 流体力学 

分 类 号：R318.01[医药卫生—生物医学工程]