裂皮病病毒在柑橘不同部位的分布研究  

The Distribution of Citrus Exocortis Viroid in Citrus

作　　者：谢金招[1,2] 果顺 何智强 陈扬平 曾芳群 陈红丽 胡珊玲[1,3] 刘永泉[1,3] XIE Jinzhao;GUO Shun;HE Zhiqiang;CHEN Yangping;ZENG Fangqun;CHEN Hongli;HU Shanling;LIU Yongquan(National Navel Orange Engineering Research Center,Gannan Normal University,Ganzhou 341000,China;Ganzhou Fruit Industry Development Center;College of Life Sciences,Gannan Normal University,Ganzhou 341000,China)

机构地区：[1]赣南师范大学国家脐橙工程技术研究中心,江西赣州341000 [2]赣州市果业发展中心 [3]赣南师范大学生命科学学院,江西赣州341000

出　　处：《赣南师范大学学报》2024年第3期48-51,共4页Journal of Gannan Normal University

基　　金：江西省自然科学基金(20232BAB215006);江西省教育厅科技项目(GJJ2201257);赣南师范大学博士科研启动基金(BSJJ202254)。

摘　　要：柑橘裂皮病类病毒(Citrus exocortis viroid,CEVd)主要为害以枳和枳橙作砧木的柑橘品种,引起砧木树皮开裂、植株矮化,导致病树枯枝、落花落果,严重时可致全株死亡,严重阻碍了柑橘的优质生产.前期研究发现,柑橘样品的采集部位对CEVd检测具有显著影响.为进一步探究不同部位样品中CEVd滴度情况及对检测的影响,从感染柑橘裂皮病的白金柚和脐橙植株上分别取嫩叶、成熟叶、老叶及不同年龄枝条的树皮提取总RNA,进行一步法反转录多聚酶链式反应(RT-PCR)检测,分析CEVd在柑橘体内的分布情况.结果表明成熟叶、老叶和树皮都可以检测到CEVd特异性亮条带,但是嫩叶中CEVd滴度低,容易造成检测结果的假阴性.Citrus exocortis viroid(CEVd)is a graft-transmitted virus that mainly affects citrus varieties grafted on Poncirus trifoliata(L.)Raf.and Poncirus trifoliata(L.)Raf.cv.Citrange rootstocks.It causes bark scaling and plant dwarfing,resulting in dead branches,falling flowers and fruit of diseased trees,and death of the whole plant,which seriously hinters the production of citrus industry.Previous studies have found that the collection spot of samples has a significant impact on CEVd detection.To further investigate the titer of CEVd in different parts and its impact on detection,this study extracted total RNA from tender leaves,mature leaves,old leaves,and barks of different ages of Citrus Oroblanco and C.sinensis infected with CEVd,and performed one-step reverse transcription polymerase chain reaction(RT-PCR)to analyze the distribution of CEVd in citrus.The results indicated that CEVd can be detected in mature leaves,old leaves,and bark,while the low titer of CEVd in tender leaves can easily lead to false negative.

关 键 词：Citrus exocortis viroid RT-PCR 植物病害检测 病菌分布 

分 类 号：S41[农业科学—植物保护] S666